Mahmoud Ghada Abd-Elmonsef, Bashandy Shymaa Ryhan
Botany and Microbiology Department, Faculty of Science, Assiut University, Assiut, 71516, Egypt.
Curr Microbiol. 2021 Apr;78(4):1577-1589. doi: 10.1007/s00284-021-02376-1. Epub 2021 Mar 6.
By increasing the environmental pollution, crop losses, and side effects of chemically synthesized vitamins; new vitamin sources should be included. Through this study, we introduce novel riboflavin bacterial producer Novosphingobium panipatense-SR3 (MT002778) and tested various nutritional factors with interactions effects on the production abilities. Yeast extract, maltose, and glycine were the best nitrogen, carbon, and amino acid sources for enhancing the production, respectively. The interaction between the previous factors with three concentrations of each (+, 0, -) studied statistically using Box-Behnken statistical quadric design 13- run. The perfect interaction increases the production to 497.12 mg/l (predicted 489.45 mg/l) using 30 g/l maltose, 10 g/l yeast extract, and 1 g/l glycine. The F and P- values of the tested model of riboflavin and OD600 indicating significant results with probability ≤ 0.05. Also, the evaluating statistical parameter coefficient (R) was 0.994 of riboflavin and 0.992 of OD600 with adjusted R value 0.976, and 0.967, respectively, which indicated that the whole variations were explained highly by the statistical model. The novel producer proved its high riboflavin production ability especially under the optimized conditions comparing with previous producers and represents a new high-speed riboflavin producer that could utilize in the industrial process.
由于环境污染加剧、作物损失以及化学合成维生素的副作用，应纳入新的维生素来源。通过本研究，我们引入了新型核黄素细菌生产者潘尼帕特新鞘氨醇菌 - SR3（MT002778），并测试了各种营养因素及其对生产能力的相互作用影响。酵母提取物、麦芽糖和甘氨酸分别是提高产量的最佳氮源、碳源和氨基酸源。使用Box - Behnken统计二次设计进行13次运行，对每种三种浓度（+、0、 - ）的上述因素之间的相互作用进行了统计研究。使用30 g/l麦芽糖、10 g/l酵母提取物和1 g/l甘氨酸时，完美的相互作用使产量提高到497.12 mg/l（预测值489.45 mg/l）。核黄素和OD600测试模型的F值和P值表明结果具有显著性，概率≤0.05。此外，评估统计参数系数（R）对于核黄素为0.994，对于OD600为0.992，调整后的R值分别为0.976和0.967，这表明统计模型高度解释了整体变化。与先前的生产者相比，这种新型生产者在优化条件下尤其证明了其高产核黄素的能力，并且代表了一种可用于工业生产过程的新型高产核黄素生产者。
