Tao Yong, Li Guo-Hui, Hu Yu-Ping, Mekki M Dafalla, Chen Kuan-Wei, Wang Jin-Yu
Animal Science and Technology Department of Yangzhou University, Yangzhou 225009, China.
Yi Chuan. 2008 Jul;30(7):900-6. doi: 10.3724/sp.j.1005.2008.00900.
Melanocortin-4 receptor (MC4R) gene and pituitary specific transcription factor 1 (POU1F1) gene were studied as candidate genes on the Jinghai yellow chicken in this study. Polymorphisms of MC4R and POU1F1 gene had been analyzed by PCR-SSCP and DNA sequencing method. The relationship between two candidate genes and the growth performance of Jinghai yellow chicken had also been analyzed by GLM model. The frequency of A/B alleles for MC4R gene in the population was 0.929 and 0.071, and that of C/D alleles for POU1F1 gene was 0.500 and 0.500. The results showed that AA genotype of MC4R gene had higher bodyweight at 4, 8, 12 weeks compared to AB and BB genotype (P<0.05); CD genotype of POU1F1 gene had significantly higher bodyweight at all weeks compared to CC and DD genotype (P<0.01). So, it could be preliminarily deduced that MC4R and POU1F1 gene were probably the major gene on growth performance or a QTL linked gene which associated with growth performance in chicken.
本研究将黑皮质素-4受体（MC4R）基因和垂体特异性转录因子1（POU1F1）基因作为京海黄鸡的候选基因进行研究。采用PCR-SSCP和DNA测序方法分析了MC4R和POU1F1基因的多态性。同时利用GLM模型分析了这两个候选基因与京海黄鸡生长性能之间的关系。群体中MC4R基因A/B等位基因的频率分别为0.929和0.071，POU1F1基因C/D等位基因的频率均为0.500。结果表明，MC4R基因AA基因型在4、8、12周龄时的体重显著高于AB和BB基因型（P<0.05）；POU1F1基因CD基因型在各周龄时的体重均显著高于CC和DD基因型（P<0.01）。因此，可以初步推断MC4R和POU1F1基因可能是影响鸡生长性能的主基因或与生长性能相关的QTL连锁基因。
