Jiang Mei-Shan, Chen Shi-Yi, Lai Song-Jia, Deng Xiao-Song, Chen Yun, Wan Jie
College of Animal Science and Technology, Sichuan Agricultural University, Ya'an 625014, China.
Yi Chuan. 2008 Dec;30(12):1574-8. doi: 10.3724/sp.j.1005.2008.01574.
Single nucleotide polymorphisms in the coding sequence of melanoeortin-4 receptor (MC4R) gene were detected by PCR-SSCP and DNA sequencing method in Harbin white rabbit, Tianfu black rabbit, Belgian hare, ZIKA rabbit, and California rabbit breeds. A-->G conversion mutation at base position 237 was found with high frequency in Harbin white rabbit, Belgian hare, and Zika rabbit and low frequency in Tianfu black rabbit and California rabbit. The allele A was pre-dominant allele for each of meat rabbit breeds. AA genotype frequency was higher than AG genotype in the five studied rabbit breeds. GLM analysis for the effect of genotypes on performance traits demonstrated that AG genotype was significantly associated with body weight, eviscerated weight and feed conversion efficiency (P<0.05), but not significantly associated with cooking loss (P>0.05). It was concluded from the results that MC4R gene could be a candidate modifier gene that affects or controls body weight and carcass traits of rabbit.
采用聚合酶链式反应-单链构象多态性（PCR-SSCP）和DNA测序方法，对哈尔滨白兔、天府黑兔、比利时野兔、齐卡兔和加利福尼亚兔5个品种肉兔黑素皮质素4受体（MC4R）基因编码序列中的单核苷酸多态性进行检测。结果发现，237位碱基处的A→G转换突变在哈尔滨白兔、比利时野兔和齐卡兔中出现频率较高，在天府黑兔和加利福尼亚兔中出现频率较低。等位基因A是各肉兔品种的优势等位基因。在5个供试兔品种中，AA基因型频率均高于AG基因型。基因型对生产性能影响的广义线性模型（GLM）分析表明，AG基因型与体重、胴体重和饲料转化率显著相关（P<0.05），但与熟肉率无显著关联（P>0.05）。研究结果表明，MC4R基因可能是影响或控制肉兔体重和胴体性状的候选修饰基因。
