Zeng Xue, Yang Zu-Jun, Li Guang-Rong, Lei Meng-Ping, Liu Cheng, Jia Ju-Qing, Ren Zheng-Long
School of Life Science and Technology, University of Electronic Science and Technology of China, Chengdu 610054, China.
Yi Chuan. 2008 Aug;30(8):1056-62. doi: 10.3724/sp.j.1005.2008.01056.
Genome in situ hybridization (GISH) analysis of wheat-Secale africanum amphiploid revealed that the S. africanum genome displayed significant divergence to the Secale cereale genome. It is thus valuable to deploy genes from S. africanum. We performed the PCR analysis on S. africanum, wheat-S. afticanum amphiploid, T. eastivum cv. Anyuepaideng and other genetic stocks by 100 ISSR primers. A specific segment of 561 bp, named pSaUBC815561, was obtained from S. africanum using primer UBC815. This segment was not amplified from the control wheat lines. Primer UBC815 also am-plified fragments from wild species of genus Secale, including S. vavilovii, S. sylvestre, and other cultivated ryes. Based on the sequence of pSaUBC815561, a pair of special primers U815-F and U815-R was designed and was used to amplify the DNA of wheat related species in Triticeae aimed at validating the specificity of pSaUBC815561. PCR analysis indicated that this specific DNA fragment was amplified not only from a set of Chinese Spring wheat-Imperial rye chromosome addition lines but also from certain wheat-rye introgression lines. Therefore, pSaUBC815561 can be used as a specific marker for detection of chromosomes of Secale genome in wheat.
对小麦-非洲黑麦双二倍体进行基因组原位杂交（GISH）分析表明，非洲黑麦基因组与黑麦基因组存在显著差异。因此，利用非洲黑麦的基因具有重要价值。我们用100条ISSR引物对非洲黑麦、小麦-非洲黑麦双二倍体、普通小麦品种安岳矮登等遗传材料进行了PCR分析。使用引物UBC815从非洲黑麦中获得了一个561 bp的特异性片段，命名为pSaUBC815561。对照小麦品系未扩增出该片段。引物UBC815还从黑麦属的野生种，包括瓦维洛夫黑麦、森林黑麦和其他栽培黑麦中扩增出片段。根据pSaUBC815561的序列，设计了一对特异性引物U815-F和U815-R，用于扩增小麦族中小麦近缘种的DNA，以验证pSaUBC815561的特异性。PCR分析表明，这个特异性DNA片段不仅能从一套中国春小麦-帝王黑麦染色体附加系中扩增出来，也能从某些小麦-黑麦渐渗系中扩增出来。因此，pSaUBC815561可作为检测小麦中黑麦基因组染色体的特异性标记。
