González Cecilia, Camacho Manuel V, Benito César
Laboratory of Genetics, Universidad San Pablo, CEU, Madrid, Spain.
Genetica. 2002 Jun;115(2):205-11. doi: 10.1023/a:1020176926378.
The polymerase chain reaction (PCR) was used to locate RAPD markers using disomic wheat-rye addition lines in order to develop a set of molecular markers distributed on the seven rye chromosomes. We carried out RAPD amplifications on genomic DNA of wheat 'Chinese Spring' (CS), rye 'Imperial' (I), the amphiploid wheat-rye and the seven disomic wheat-rye addition lines (1R-7R) using 140 different 10-mer oligonucleotides. Forty six new RAPD markers were located on the seven rye chromosomes and all the disomic wheat-rye addition lines were identified on the basis of their amplification patterns. The number of RAPD bands located on 1R, 2R, 3R, 4R, 5R, 6R and 7R chromosomes were 5, 8, 11, 8, 8, 10 and 6, respectively. The seven wheat-rye addition lines can be distinguished using only the following three 10-mer oligonucleotides: OPA16, OPF19 and GEN3-605, the other RAPD primers being useful for this purpose. The use of these RAPDs as a source of molecular markers that could be linked to interesting genes or other important agronomic traits is discussed.
为了开发一套分布在七条黑麦染色体上的分子标记，利用二体小麦-黑麦附加系，通过聚合酶链式反应（PCR）定位随机扩增多态性DNA（RAPD）标记。我们使用140种不同的10聚体寡核苷酸，对小麦“中国春”（CS）、黑麦“帝国”（I）、小麦-黑麦双二倍体以及七个二体小麦-黑麦附加系（1R-7R）的基因组DNA进行RAPD扩增。46个新的RAPD标记定位在七条黑麦染色体上，并根据扩增模式鉴定了所有的二体小麦-黑麦附加系。位于1R、2R、3R、4R、5R、6R和7R染色体上的RAPD条带数分别为5、8、11、8、8、10和6。仅使用以下三种10聚体寡核苷酸：OPA16、OPF19和GEN3-605，就可以区分这七个小麦-黑麦附加系，其他RAPD引物也可用于此目的。本文还讨论了将这些RAPD作为与感兴趣基因或其他重要农艺性状连锁的分子标记来源的用途。
