Hao Rong-Chao, Zan Lin-Sen, Liu Chou-Sheng, Wang Zhi-Gang, Zhang Gui-Xiang, Han Xu, Hao Huai-Zhi, Wang Jing, DU Xiao-Yong
College of Animal Science and Technology, Northwest A&F University, Yangling 712100, China.
Yi Chuan. 2008 Sep;30(9):1187-94. doi: 10.3724/sp.j.1005.2008.01187.
The complete sequences of mitochondrial DNA D-loop of 140 individuals in 10 Chinese goat (Capra hiruc) breeds were analyzed by DNA sequencing technology. The results showed that the length of mtDNA D-loop in Chinese goats was 1 211-1 213 bp. There were 84 different haplotypes and 171 polymorphic sites. The mean nucleotide diversity (Pi) and haplotype diversity (Hd) were 0.02063+/+0.00225 and 0.988+/-0.003, respectively, and the average number of nucleotide differences (k) was 24.896. The results showed an abundant genetic diversity in domestic goats of China. The NJ tree indicated that there were two main branches in Chinese domestic goats, thereinto, one branch was clustered with Capra aegagrus, and Capra falconeri was clustered alone, which indicated that Capra aegagrus had more contribution to Chinese domestic goat breeds.
采用DNA测序技术对10个中国山羊品种140个个体的线粒体DNA D-loop全序列进行了分析。结果表明,中国山羊mtDNA D-loop长度为1 211 - 1 213 bp。共有84种不同单倍型和171个多态性位点。平均核苷酸多样性(Pi)和单倍型多样性(Hd)分别为0.02063±0.00225和0.988±0.003,平均核苷酸差异数(k)为24.896。结果表明中国家山羊具有丰富的遗传多样性。NJ树显示中国家山羊主要分为两个分支,其中一个分支与角羊聚类,捻角山羊单独聚类,表明角羊对中国家山羊品种贡献较大。