线粒体DNA D环区的遗传多样性与中国山羊的起源
The genetic diversity of mtDNA D-loop and the origin of Chinese goats.
作者信息
Liu Ruo-Yu, Yang Gong-She, Lei Chu-Zhao
机构信息
College of Animal Science and Technology, Northwest A&F University, Yangling, China.
出版信息
Yi Chuan Xue Bao. 2006 May;33(5):420-8. doi: 10.1016/S0379-4172(06)60069-3.
The complete sequences of mitochondrial DNA D-loop of 128 individuals in nine Chinese goat (Capra hircu) breeds were analyzed by DNA sequencing technology. The results show that the length of mtDNA D-loop in Chinese goats is 1,212-1,213 bp. There are 102 polymorphic sites, accounting for 8.42% of 1,212 bp sequence. Ninety-two mtDNA haplotypes were determined. The haplotype diversity and nucleotide diversity are 0.9333-1.0000 and 0.7062%-1.8265%, respectively. The results indicate that the genetic diversity of Chinese goats is very abundant. The NJ tree indicates that Chinese goats have two types of maternal origins from lineage A and lineage B. The possibility of lineage B originating from China is also discussed.
采用DNA测序技术对9个中国山羊品种128个个体的线粒体DNA D环完整序列进行了分析。结果表明，中国山羊mtDNA D环长度为1212 - 1213 bp。共有102个多态性位点，占1212 bp序列的8.42%。确定了92种mtDNA单倍型。单倍型多样性和核苷酸多样性分别为0.9333 - 1.0000和0.7062% - 1.8265%。结果表明，中国山羊的遗传多样性非常丰富。NJ树表明中国山羊有来自A系和B系的两种母系起源类型。还讨论了B系起源于中国的可能性。
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