Cope C, Delbridge L, Philips J, Friedlander M
Endocrine Surgical Unit, Royal North Shore Hospital, St Leonards, New South Wales, Australia.
Aust N Z J Surg. 1991 Sep;61(9):695-8. doi: 10.1111/j.1445-2197.1991.tb00323.x.
Nuclear DNA content of 27 phaeochromocytomas was measured by image analysis of thin sections and monolayer smears of nuclei extracted from thick sections (cytospins) using archival paraffin-embedded tissue blocks. Recurrence was assessed on the basis of clinical follow-up or urinary catecholamine levels. The mean follow-up was 5.0 years, with a range of 4 months to 15 years. Although it is not possible to differentiate accurately malignant tumours using DNA analysis, prediction of benign tumours is possible. Using thin sections, all tumours were benign when the percentage of cells with a DNA content of 2.5 c (2 c = diploid) was less than 40% and the DNA content of 5 c was less than 4%. Using the cytospin preparation, all diploid tumours were benign. Thus, patients with diploid tumours may be saved intensive long-term follow-up. Aneuploid and tetraploid tumours, however, continue to require careful lifelong follow-up.
利用存档石蜡包埋组织块，通过对从厚切片（细胞离心涂片）中提取的细胞核的薄片和单层涂片进行图像分析，测量了27例嗜铬细胞瘤的核DNA含量。根据临床随访或尿儿茶酚胺水平评估复发情况。平均随访时间为5.0年，范围为4个月至15年。虽然使用DNA分析不可能准确区分恶性肿瘤，但可以预测良性肿瘤。使用薄片时，当DNA含量为2.5c（2c = 二倍体）的细胞百分比小于40%且5c的DNA含量小于4%时，所有肿瘤均为良性。使用细胞离心涂片制备时，所有二倍体肿瘤均为良性。因此，二倍体肿瘤患者可能无需长期密集随访。然而，非整倍体和四倍体肿瘤仍需要终身仔细随访。
