嗜铬细胞瘤的脱氧核糖核酸流式细胞术研究及其与临床参数的相关性。
Deoxyribonucleic acid flow cytometric study in pheochromocytomas and its correlation with clinical parameters.
作者信息
Lai M K, Sun C F, Chen C S, Huang C C, Chu S H, Chuang C K
机构信息
Department of Surgery, Chang Gung Memorial Hospital, Chang Gung Medical College, Taipei, Taiwan.
出版信息
Urology. 1994 Aug;44(2):185-8. doi: 10.1016/s0090-4295(94)80126-6.
OBJECTIVES
To study deoxyribonucleic acid flow cytometry (DNA-FC) in pheochromocytomas and its correlation with clinical parameters.
METHODS
Fifty cases of pheochromocytomas of a total of 58 cases were studied for DNA-FC from archival paraffin-embedded tissue blocks and were correlated with clinical parameters (ie, size of tumor, benign or malignant pathologic type, vanillymandelic acid [VMA] level in 24 hour urine, age, sex, duration of symptoms, symptom scores).
RESULTS
A total of 19 cases were diploid while 31 cases (62%) showed DNA aneuploidy with 21 cases tetraploid. Four cases were malignant. All of them were tetraploid. However, among the 46 benign cases, 10 were aneuploid and 17 were tetraploid. All 19 cases with DNA diploidy were benign. The DNA-FC did not correlate with other clinical parameters in our study.
CONCLUSIONS
We confirmed a high incidence of DNA aneuploidy in pheochromocytomas. All malignant pheochromocytomas were tetraploid and all cases with DNA diploidy were benign. Long-term follow-up of all cases, especially those with DNA aneuploidy (including tetraploidy), is mandatory.
目的
研究嗜铬细胞瘤的脱氧核糖核酸流式细胞术（DNA-FC）及其与临床参数的相关性。
方法
对58例嗜铬细胞瘤中的50例进行研究，从存档石蜡包埋组织块中提取DNA-FC，并与临床参数（即肿瘤大小、良恶性病理类型、24小时尿香草扁桃酸[VMA]水平、年龄、性别、症状持续时间、症状评分）进行相关性分析。
结果
共有19例为二倍体，31例（62%）显示DNA非整倍体，其中21例为四倍体。4例为恶性，均为四倍体。然而，在46例良性病例中，10例为非整倍体，17例为四倍体。所有19例DNA二倍体病例均为良性。在我们的研究中，DNA-FC与其他临床参数无相关性。
结论
我们证实嗜铬细胞瘤中DNA非整倍体的发生率很高。所有恶性嗜铬细胞瘤均为四倍体，所有DNA二倍体病例均为良性。对所有病例，尤其是DNA非整倍体（包括四倍体）病例进行长期随访是必要的。
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