Zare Ahad, Farzaneh Parvaneh, Pourpak Zahra, Zahedi Fatemeh, Moin Mostafa, Shahabi Shahram, Hassan Zuhair M
Department of Immunology, School of Medical Sciences, Tarbiat Modares University, Tehran, Iran.
Iran J Allergy Asthma Immunol. 2008 Sep;7(3):133-41.
Garlic is known as a potent spice and a medicinal herb with broad therapeutic properties ranging from antibacterial to anticancer and anticoagulant. Our previous studies have shown some immunoregulatory effects for aged garlic extract, suggesting a key role for 14-kD glycoprotein of garlic in shifting the cytokine pattern to T helper-1. In present study, we investigated the effect of 1, 2, and 3 times intraperitoneal injections of aged garlic extract on an established allergic airway inflammation in murine model (BALB/c mice). The garlic extract, isolated by biochemical method, includes proteins precipitation by ammonium sulfate. After injection of the aged garlic extract, IFN-, anti allergen specific IgE and IgG1 were measured in lavage and serum by ELISA and histological assessment was performed on the lung tissues. The results indicated that three-time intra peritoneal injections of the aged garlic extract caused a significant decrease in the hallmark criteria of allergic airway inflammation levels which included eosinophil percentage in lavage, peribronchial lung eosinophils, IgG1 level in lavage and serum, mucous producing goblet cells grade and peribronchial and perivascular inflammation. Our findings in the present research suggested that aged garlic extract has the potential of attenuation of inflammatory features of allergic airway inflammation in murine model.
大蒜作为一种强效香料和药草，具有广泛的治疗特性，从抗菌到抗癌和抗凝血。我们之前的研究表明，陈年大蒜提取物具有一些免疫调节作用，这表明大蒜的14-kD糖蛋白在将细胞因子模式转变为辅助性T细胞1型方面起着关键作用。在本研究中，我们研究了腹腔注射1次、2次和3次陈年大蒜提取物对小鼠模型（BALB/c小鼠）已建立的过敏性气道炎症的影响。通过生化方法分离的大蒜提取物包括硫酸铵沉淀的蛋白质。注射陈年大蒜提取物后，通过酶联免疫吸附测定法（ELISA）在灌洗液和血清中测量干扰素-γ、抗变应原特异性免疫球蛋白E（IgE）和免疫球蛋白G1（IgG1），并对肺组织进行组织学评估。结果表明，腹腔注射3次陈年大蒜提取物可使过敏性气道炎症水平的标志性标准显著降低，这些标准包括灌洗液中的嗜酸性粒细胞百分比、支气管周围肺嗜酸性粒细胞、灌洗液和血清中的IgG1水平、产生黏液的杯状细胞分级以及支气管周围和血管周围炎症。我们在本研究中的发现表明，陈年大蒜提取物具有减轻小鼠模型中过敏性气道炎症炎症特征的潜力。
