Yang Eun Ju, Lee Ji-Sook, Yun Chi-Young, Kim Joo-Hwan, Kim Jin-Sook, Kim Dong-Hee, Kim In Sik
Department of Biomedical Laboratory Science, School of Medicine, Eulji University, Daejeon, Republic of Korea.
J Ethnopharmacol. 2008 Jun 19;118(1):102-7. doi: 10.1016/j.jep.2008.03.012. Epub 2008 Mar 25.
Duchesnea chrysantha (D. chrysantha) is a herb with anti-oxidative, anti-inflammatory and immune-enhancing properties.
Asthma is an inflammatory disease of the lungs, and the hallmarks of the disease are increased inflammatory cell infiltration into the airways and poor respiratory function. Although there is the possibility that D. chrysantha may have an inhibitory effect on lung inflammation, the effects of D. chrysantha on asthma have not been fully investigated. In the present study, we investigated the anti-inflammatory activity of D. chrysantha extract (Dc extract) on lung inflammation in a murine model of ovalbumin-induced asthma.
Dc extract was obtained from dried and powdered whole plants of D. chrysantha using 80% ethanol. BALB/c mice induced by ovalbumin sensitization and nebulization were used as a mouse model of asthma. RT-PCR and ELISA were performed to measure mRNA and protein expression of cytokines. We examined the effects of Dc extract on leukocyte infiltration and mucus secretion using periodic acid-Schiff staining as well as hematoxylin and eosin staining.
Dc extract significantly inhibited leukocytosis and eosinophilia in the bronchoalveolar lavage (BAL) fluid (p<0.01). Dc extract significantly reduced the elevated infiltration of inflammatory cells (p<0.05) and inhibited the increased mucus secretion, despite the absence of significant value. Although Dc extract weakly inhibited the mRNA expression of IL-4, IL-5, IL-13, and eotaxin, it strongly inhibited the protein expression of IL-5 (p<0.05) and eotaxin (p<0.01) in BAL fluid. Ovalbumin-specific IgE levels in the serum and BAL fluid were blocked by Dc extract (p<0.05).
These results suggest the possibility that Dc extract can exert suppressive effects on asthma and may provide evidence that Dc extract is a useful agent for the treatment of allergic airway disease.
蛇莓是一种具有抗氧化、抗炎和免疫增强特性的草药。
哮喘是一种肺部炎症性疾病，其特征是气道内炎症细胞浸润增加和呼吸功能不佳。尽管蛇莓可能对肺部炎症有抑制作用，但蛇莓对哮喘的影响尚未得到充分研究。在本研究中，我们研究了蛇莓提取物（Dc提取物）对卵清蛋白诱导的哮喘小鼠模型肺部炎症的抗炎活性。
使用80%乙醇从干燥粉碎的蛇莓全株中获得Dc提取物。通过卵清蛋白致敏和雾化诱导的BALB/c小鼠用作哮喘小鼠模型。进行RT-PCR和ELISA以测量细胞因子的mRNA和蛋白质表达。我们使用过碘酸-希夫染色以及苏木精和伊红染色检查了Dc提取物对白细胞浸润和黏液分泌的影响。
Dc提取物显著抑制支气管肺泡灌洗（BAL）液中的白细胞增多和嗜酸性粒细胞增多（p<0.01）。Dc提取物显著减少炎症细胞浸润增加（p<0.05）并抑制黏液分泌增加，尽管差异无统计学意义。尽管Dc提取物对IL-4、IL-5、IL-13和嗜酸性粒细胞趋化因子的mRNA表达有微弱抑制作用，但它强烈抑制BAL液中IL-5（p<0.05）和嗜酸性粒细胞趋化因子（p<0.01）的蛋白质表达。血清和BAL液中卵清蛋白特异性IgE水平被Dc提取物阻断（p<0.05）。
这些结果表明Dc提取物可能对哮喘发挥抑制作用，并可能提供证据表明Dc提取物是治疗过敏性气道疾病的有用药物。
