1,2-萘醌诱导白内障。I. 牛晶状体蛋白氧化还原特性的研究。

Cataract induction by 1,2-naphthoquinone. I. Studies on the redox properties of bovine lens proteins.

作者信息

Kleber E, Kröner R, Elstner E F

机构信息

Institut für Botanik und Mikrobiologie, Technische Universität München, Bundesrepublik Deutschland.

出版信息

Z Naturforsch C J Biosci. 1991 Mar-Apr;46(3-4):280-4. doi: 10.1515/znc-1991-3-419.

DOI:10.1515/znc-1991-3-419

PMID:1878111

Abstract

Conditions of oxidative stress may lead to cataract formation. Reaction of certain flavoproteins, the NADH: oxidoreductases, with different quinones is well known to form hydrogen-peroxide. This reaction was investigated to get more information on cataract induction by naphthalene and its quinone metabolites. Protein extracts from bovine lens cortex exhibit "diaphorase" activity, indicated as dye reduction in the presence of NADH and dichlorophenol-indophenol (DCPIP) or ferricyanide. Different redox cycling compounds are shown to be active in this "diaphorase" reaction by lens protein extract (LCE): Oxygen consumption can be detected in the presence of pyrroloquinoline quinone and juglone whereas 1,4-naphthoquinone, menadione and paraquat are no redox cyclists in this flavoprotein catalyzed reaction.

摘要

氧化应激状态可能导致白内障形成。某些黄素蛋白（NADH：氧化还原酶）与不同醌类的反应众所周知会生成过氧化氢。对该反应进行了研究，以获取更多关于萘及其醌类代谢产物诱导白内障的信息。牛晶状体皮质的蛋白质提取物表现出“黄递酶”活性，表现为在存在NADH和二氯酚靛酚（DCPIP）或铁氰化物的情况下染料还原。不同的氧化还原循环化合物在晶状体蛋白提取物（LCE）的这种“黄递酶”反应中显示出活性：在存在吡咯喹啉醌和胡桃醌的情况下可检测到氧气消耗，而1,4 - 萘醌、甲萘醌和百草枯在这种黄素蛋白催化的反应中不是氧化还原循环剂。