Simmons N L, Brown C D
Department of Physiological Sciences, Medical School, University of Newcastle upon Tyne.
Exp Physiol. 1991 May;76(3):457-60. doi: 10.1113/expphysiol.1991.sp003512.
Arginine vasopressin (AVP) at a concentration of 0.1 microM stimulates an inward short-circuit current (SCC) in voltage-clamped Madin-Darby canine kidney epithelial layers with a mean peak of 3.1 +/- 0.8 microA cm-2 (n = 6). The response to AVP is markedly attenuated when inward SCC is stimulated with forskolin or prostaglandin E1, in Cl(-)-free media and by the Cl(-)-transport inhibitors, bumetanide and 5-nitro-2-(3-phenylpropylamino)-benzoic acid, consistent with an AVP-stimulated basal to apical Cl- secretion. Endogenous release of prostaglandins may contribute to AVP stimulation of Cl- secretion.
浓度为0.1微摩尔的精氨酸加压素（AVP）可刺激电压钳制的Madin-Darby犬肾上皮层产生内向短路电流（SCC），平均峰值为3.1±0.8微安/平方厘米（n = 6）。当用毛喉素或前列腺素E1、在无氯培养基中以及用氯转运抑制剂布美他尼和5-硝基-2-（3-苯丙基氨基）苯甲酸刺激内向SCC时，对AVP的反应明显减弱，这与AVP刺激的从基底到顶端的氯分泌一致。前列腺素的内源性释放可能有助于AVP对氯分泌的刺激。
