Redman T, Jacobs T
National Center for Supercomputing Applications, Urbana-Champaign.
Biotechniques. 1991 Jun;10(6):790-4.
We present GelReader 1.0, a microcomputer program designed to make precision, digital analysis of one-dimensional electrophoretic gels accessible to the molecular biology laboratory of modest means. Images of electrophoretic gels are digitized via a desktop flatbed scanner from instant photographs, autoradiograms or chromogenically stained blotting media. GelReader is then invoked to locate lanes and bands and generate a report of molecular weights of unknowns, based on specified sets of standards. Frequently used standards can be stored in the program. Lanes and bands can be added or removed, based upon users' subjective preferences. A unique lane histogram feature facilitates precise manual addition of bands missed by the software. Image enhancement features include palette manipulation, histogram equalization, shadowing and magnification. The user interface strikes a balance between program autonomy and user intervention, in recognition of the variability in electrophoretic gel quality and users' analytical needs.
我们展示了GelReader 1.0，这是一个微型计算机程序，旨在让资源有限的分子生物学实验室能够对一维电泳凝胶进行精确的数字分析。电泳凝胶的图像通过台式平板扫描仪从即时照片、放射自显影片或显色染色的印迹介质中进行数字化处理。然后调用GelReader来定位泳道和条带，并根据指定的标准集生成未知物分子量的报告。常用标准可以存储在程序中。可以根据用户的主观偏好添加或删除泳道和条带。独特的泳道直方图功能有助于精确手动添加软件遗漏的条带。图像增强功能包括调色板操作、直方图均衡化、阴影和放大。用户界面在程序自主性和用户干预之间取得了平衡，以认识到电泳凝胶质量的变异性和用户的分析需求。
