Häder D P, Truss M
Fachbereich Biologie--Botany and Genetics, Marburg, FRG.
Comput Appl Biosci. 1987 Nov;3(4):339-43.
A low cost, microcomputer-controlled image analysis system is described which scans electrophoresis gels or photographic negatives with high resolution. Absorbing gels (e.g. with stained proteins) are analyzed using broadband or monochromatic visible light. The gel is scanned by a video camera with a macro objective; the gray level of each pixel is digitized sequentially and the values are stored in the computer memory. Repetitive scanning is used to average the absorption values before plotting on a digital plotter. Photographic negatives of gels and autoradiograms are scanned using the same technique. Fluorescent, non-absorbing gels (e.g. nucleic acids stained with ethidium bromide) are analyzed on a transilluminator with u.v. excitation radiation. The fluorescence intensity of each pixel is digitized and processed as described above.
本文描述了一种低成本的、由微型计算机控制的图像分析系统，该系统能以高分辨率扫描电泳凝胶或照相底片。对于吸收性凝胶（如含有染色蛋白质的凝胶），使用宽带或单色可见光进行分析。凝胶由配有微距物镜的摄像机进行扫描；每个像素的灰度值被依次数字化，其数值存储在计算机内存中。在将吸收值绘制到数字绘图仪上之前，使用重复扫描来平均这些值。凝胶和放射自显影片的照相底片采用相同技术进行扫描。对于荧光性、非吸收性凝胶（如用溴化乙锭染色的核酸），在配有紫外激发辐射的透照仪上进行分析。每个像素的荧光强度被数字化并按上述方法进行处理。
