Scholz Christoph, Toth Bettina, Santoso Laura, Kuhn Christina, Franz Maximilian, Mayr Doris, Jeschke Udo, Friese Klaus, Schiessl Barbara
Department of Obstetrics and Gynecology - Maistrasse, Ludwig-Maximilians University, Munich, Germany.
Am J Reprod Immunol. 2008 Sep;60(3):238-45. doi: 10.1111/j.1600-0897.2008.00619.x.
The immunological equilibrium at the feto-maternal interphase contributes towards late gestational diseases like growth restriction (IUGR) pre-eclampsia (PE) and hemolysis, elevated liver enzymes, low platelets (HELLP)-syndrome. The state of activation of decidual dendritic cells (DC) has emerged as one of the central players influencing this immunological equilibrium.
Paraffin-embedded tissue sections from 27 pregnancies were immunostained for DC markers DEC-205, DC-SIGN, DC-LAMP and costained for DC-SIGN/CD56 and DC-SIGN/ vascular endothelial growth factor receptor (VEGFR) -1 and -2. We investigated placental tissue of IUGR fetuses and of patients who developed PE or HELLP-syndrome as well as placental tissue derived from normal pregnancies.
We found that expression of DEC-205 and DC-SIGN was significantly upregulated in HELLP placentas, whereas expression of DC-LAMP was abrogated almost entirely. Costaining showed an interaction between DC-SIGN(+) DC and natural killer cells as well as costaining of VEGFR-1 and -2 and DC-SIGN. Pre-eclamptic and IUGR placentas showed no significant change in any of the investigated markers compared to normal controls.
Our data suggest a participation of DC-mediated immunological mechanisms in HELLP syndrome.
母胎界面的免疫平衡与诸如胎儿生长受限（IUGR）、先兆子痫（PE）和溶血、肝酶升高、血小板减少（HELLP）综合征等妊娠晚期疾病有关。蜕膜树突状细胞（DC）的激活状态已成为影响这种免疫平衡的核心因素之一。
对27例妊娠的石蜡包埋组织切片进行DC标志物DEC-205、DC-SIGN、DC-LAMP免疫染色，并对DC-SIGN/CD56以及DC-SIGN/血管内皮生长因子受体（VEGFR）-1和-2进行共染色。我们研究了IUGR胎儿、发生PE或HELLP综合征患者的胎盘组织以及正常妊娠的胎盘组织。
我们发现，DEC-205和DC-SIGN的表达在HELLP胎盘组织中显著上调，而DC-LAMP的表达几乎完全消失。共染色显示DC-SIGN(+) DC与自然杀伤细胞之间存在相互作用，同时VEGFR-1和-2与DC-SIGN也存在共染色。与正常对照组相比，先兆子痫和IUGR胎盘组织中任何一项研究标志物均无显著变化。
我们的数据表明DC介导的免疫机制参与了HELLP综合征。
