醋酸酯衍生物在气相色谱-质谱联用中的应用：糖类、脂质和氨基酸分析的新方法

Application of acetate derivatives for gas chromatography-mass spectrometry: novel approaches on carbohydrates, lipids and amino acids analysis.

作者信息

Sassaki Guilherme L, Souza Lauro M, Serrato Rodrigo V, Cipriani Thales R, Gorin Philip A J, Iacomini Marcello

机构信息

Departamento de Bioquímica e Biologia Molecular, Universidade Federal do Paraná, CP 19046, Curitiba, Paraná, CEP 81531-980, Brazil.

出版信息

J Chromatogr A. 2008 Oct 24;1208(1-2):215-22. doi: 10.1016/j.chroma.2008.08.083. Epub 2008 Aug 28.

DOI:10.1016/j.chroma.2008.08.083

PMID:18783777

Abstract

The structure of glycoconjugates has been determined by several chromatographic methods, however gas chromatography-mass spectrometry (GC-MS) has been widely used to identify and quantify the volatile trimethylsilyl and fluoroacyl derivatives. Adapting the reduction/acetylation strategies, we had performed the derivatization of all monosaccharide class, as well as amino acids and OH-fatty acids as from different glycoconjugates. Uronic acids gave characteristic ions at m/z 143, 156 and 173, and 19 amino acids derivatives, gave molecular ions [M]+ and daughter ions of [M-59]+ and [M-43]+ on electron impact (EI)-MS, which provide their rapid identification.

摘要

糖缀合物的结构已通过多种色谱方法确定，然而气相色谱 - 质谱联用（GC - MS）已被广泛用于鉴定和定量挥发性三甲基硅烷基和氟酰基衍生物。采用还原/乙酰化策略，我们对所有单糖类别以及来自不同糖缀合物的氨基酸和羟基脂肪酸进行了衍生化。糖醛酸在m/z 143、156、173处产生特征离子，19种氨基酸衍生物在电子轰击（EI） - MS上产生分子离子[M]+以及[M - 59]+和[M - 43]+的子离子，这有助于它们的快速鉴定。