Rajendiran Soundravally, Lakshamanappa Hoti Sugeerappa, Zachariah Bobby, Nambiar Selvaraj
Department of Molecular Biology and Bioinformatics, Vector Control Research Centre, Pondicherry, India.
Am J Trop Med Hyg. 2008 Sep;79(3):372-7.
Oxidative stress in dengue infection has been suggested. This study was carried out to explore the plasma protein oxidation and its sialic acid content in dengue infection. Thirty-two dengue hemorrhagic fever (DHF), 25 dengue shock syndrome (DSS), 29 dengue fever (DF), and 63 healthy controls were included in this study. The extent of carbonylation, sulphydryl content, and desialylation of plasma protein was estimated in acute phase sample. Significantly higher levels of protein carbonyls and lower levels of sialic acid and sulphydryl groups were found in DHF and DSS compared with DF using one-way analysis of variance. Regression analysis showed that desialylation is dependent on protein carbonyls in DHF/DSS. This study indicates that, in dengue infection, plasma proteins undergo increased levels of desialylation, which can be attributed to the oxidative stress. Future studies on sialylation status of endothelium and platelets can show light into the pathogenesis of the dengue infection.
已有研究表明登革热感染存在氧化应激。本研究旨在探讨登革热感染时血浆蛋白氧化及其唾液酸含量。本研究纳入了32例登革出血热（DHF）患者、25例登革休克综合征（DSS）患者、29例登革热（DF）患者以及63名健康对照者。在急性期样本中评估了血浆蛋白的羰基化程度、巯基含量和去唾液酸化情况。采用单因素方差分析发现，与DF患者相比，DHF和DSS患者的蛋白羰基水平显著升高，唾液酸和巯基水平降低。回归分析表明，DHF/DSS患者的去唾液酸化依赖于蛋白羰基。本研究表明，在登革热感染时，血浆蛋白的去唾液酸化水平升高，这可能归因于氧化应激。未来关于内皮细胞和血小板唾液酸化状态的研究可能有助于揭示登革热感染的发病机制。
