Soundravally R, Sankar P, Bobby Z, Hoti S L
Department of Molecular Biology and Bioinformatics, Vector Control Research Centre, Pondicherry, South India.
Platelets. 2008 Sep;19(6):447-54. doi: 10.1080/09537100802155284.
Oxidative stress in viral infections has been suggested. The study was carried out to assess the oxidative stress in the different clinical spectrums of dengue infection and to evaluate if thrombocytopenia is associated with lipid and protein oxidative injury. Twenty-seven dengue fever (DF), 32 dengue hemorrhagic fever (DHF) and 21 dengue shock syndrome (DSS) cases were studied at 3, 5 and 7 days of illness. Sixty-three healthy subjects were selected as controls. Serum protein carbonyls (PCOs), malendialdehyde (MDA) and total antioxidant status (TAS) were estimated in blood. Dengue infected individuals had significantly high levels of PCOs and MDA on the three days tested in comparison to controls. In DF cases, no significant changes in the levels of MDA and PCOs were found in course of time. However, among DHF and DSS, significant increase in MDA levels was found in the fifth and seventh day samples in comparison to their respective third day sample (P < 0.05). Using one way ANOVA, high PCOs levels were found in DSS in comparison to DF and DHF cases on all the three days tested (P < 0.001). TAS levels were found to be low among DSS on days 5 and 7 and day 7 in DHF when compared with DF cases. Correlation analysis between MDA and hematocrit revealed a significant positive association between them in DHF and DSS on day 5 (DHF r = 0.372; p = 0.024 and DSS r = 0.535; p = 0.0-01) and day 7 (DHF r = 0.412; p = 0.003 and DSS r = 0.765; p < 0.0001). There was an important negative correlation between platelet count and plasma lipid peroxidation levels among DHF and DSS on all three days tested [day 3 (DHF r = -0.392; p = 0.012 and DSS r = -0.453; p = 0.004), day 5 (DHF r = -0.592; p < 0.001 and DSS r = -0.581; p < 0.001) and day 7 (DHF r = -0.418; p = 0.001 and DSS r = -0.515; p = 0.002)]. This study concludes that an increase in oxidative stress was found in dengue viral infection. The level of oxidative stress was maximal in DSS followed by DHF and its severity was minimal in DF. The thrombocytopenia of dengue infection was associated with the extent of lipid peroxidation. Future studies might be carried out to find the role of oxidative damage in the ethiopathogenesis of thrombocytopenia and vascular leakage in dengue infection.
已有研究表明病毒感染中存在氧化应激。本研究旨在评估登革热感染不同临床谱中的氧化应激情况，并评估血小板减少症是否与脂质和蛋白质氧化损伤相关。在发病第3、5和7天，对27例登革热（DF）、32例登革出血热（DHF）和21例登革休克综合征（DSS）患者进行了研究。选取63名健康受试者作为对照。检测血液中的血清蛋白羰基（PCOs）、丙二醛（MDA）和总抗氧化状态（TAS）。与对照组相比，登革热感染个体在检测的三天中PCOs和MDA水平显著升高。在DF病例中，MDA和PCOs水平在病程中未发现显著变化。然而，在DHF和DSS患者中，与各自的第3天样本相比，第5天和第7天样本中的MDA水平显著升高（P < 0.05）。使用单因素方差分析发现，在检测的所有三天中，DSS患者的PCOs水平均高于DF和DHF病例（P < 0.001）。与DF病例相比，DSS患者在第5天和第7天以及DHF患者在第7天的TAS水平较低。MDA与血细胞比容之间的相关性分析显示，在DHF和DSS患者中，第5天（DHF r = 0.372；p = 0.024，DSS r = 0.535；p = 0.001）和第7天（DHF r = 0.412；p = 0.003，DSS r = 0.765；p < 0.0001）二者之间存在显著正相关。在检测的所有三天中，DHF和DSS患者的血小板计数与血浆脂质过氧化水平之间均存在重要的负相关[第3天（DHF r = -0.392；p = 0.012，DSS r = -0.453；p = 0.004），第5天（DHF r = -0.592；p < 0.001，DSS r = -0.581；p < 0.001）和第7天（DHF r = -0.418；p = 0.001，DSS r = -0.515；p = 0.002）]。本研究得出结论，登革热病毒感染中存在氧化应激增加。氧化应激水平在DSS中最高，其次是DHF，在DF中最低。登革热感染的血小板减少症与脂质过氧化程度相关。未来可开展研究以探究氧化损伤在登革热感染中血小板减少症和血管渗漏的发病机制中的作用。
