Guthrie J L, Seah C, Brown S, Tang P, Jamieson F, Drews S J
Ministry of Health and Long-Term Care, 81 Resources Rd., Toronto, Ontario M9P 3T1, Canada.
J Clin Microbiol. 2008 Nov;46(11):3798-9. doi: 10.1128/JCM.01551-08. Epub 2008 Sep 10.
This study utilized the Bordetella pertussis single-copy PCR target BP3385 as a means of confirming IS481 PCR-positive reactions with cycle threshold (C(T)) values of >35. IS481 PCRs with C(T) values of >35 cycles may represent PCR conditions where there is <1 CFU of B. pertussis per PCR.
本研究利用百日咳博德特氏菌单拷贝PCR靶标BP3385来确认循环阈值(C(T))值>35的IS481 PCR阳性反应。C(T)值>35个循环的IS481 PCR可能代表每PCR中百日咳博德特氏菌<1 CFU的PCR条件。