Zhang Bingbing, Pan Jun, Wang Yuanliang, Xian Chengyu, Tang Liling
Bioengineering College, Research Center of Bioinspired Materials Science and Engineering, Key Laboratory for Biomechanics & Tissue Engineering, The State Ministry of Education, Chongqing University, Chongqing 400044, China.
Sheng Wu Yi Xue Gong Cheng Xue Za Zhi. 2008 Aug;25(4):845-8.
This is an experimental study in the realm of physiology inquiring about the effect of pulsatile fluid flow shear stress on the proliferation, differentiation and functions of osteoblasts;the objective is to validate the important effect of fluid flow shear stress on the mechanics adaptability of bone tissue. The osteoblasts derived from Wistar rat's calvaria were exposed to fluid shear stress 5, 10, 20 and 30 mN/cm2 for 3, 6, 9, 12, 24, 36h respectively in the flow chamber. The ability of proliferation, alkaline phosphatase (ALP) activity and extracellular calcium secretion of osteoblasts were assessed. The results showed that fluid flow shear stress at 5, and 10 mN/cm2 increased the proliferation, but at 20 and 30 N/cm2, the shear stress inhibited the proliferation. The shear stress at 5, 10, 20 mN/cm2 increased the ALP activity and extracellular calcium secretion of osteoblasts, and advanced the time of the peak value of ALP activity during the experiment period, but the shear stress at 30 mN/cm2 decreased ALP activity. So osteoblasts responded rapidly to shear stress; the proliferation, differentiation and mineralization of cells were regulated in the presence of some shear stress; and such regulation exhibited a pattern of dependence on the mN/cm2 level of shear stress.
这是一项生理学领域的实验研究，探究脉动流体流动切应力对成骨细胞增殖、分化及功能的影响；目的是验证流体流动切应力对骨组织力学适应性的重要作用。将源自Wistar大鼠颅骨的成骨细胞分别在流动腔中暴露于5、10、20和30 mN/cm²的流体切应力下3、6、9、12、24、36小时。评估成骨细胞的增殖能力、碱性磷酸酶（ALP）活性和细胞外钙分泌。结果显示，5和10 mN/cm²的流体流动切应力增加了增殖，但在20和30 N/cm²时，切应力抑制了增殖。5、10、20 mN/cm²的切应力增加了成骨细胞的ALP活性和细胞外钙分泌，并提前了实验期间ALP活性峰值出现的时间，但30 mN/cm²的切应力降低了ALP活性。因此，成骨细胞对切应力反应迅速；在一定切应力存在下，细胞的增殖、分化和矿化受到调节；且这种调节呈现出对切应力mN/cm²水平的依赖性模式。
