Zang Hong-Min, Chen Jun-Chang, Liu Yi-Heng, Wang Kun-Zheng
Department of Orthopedics, Second Hospital of Xi'an Jiaotong University, Xi'an 710004, China.
Zhongguo Zhong Yao Za Zhi. 2005 Dec;30(24):1947-9.
To observe the effect of puerarin on cell proliferation, differentiation, maturation and mineralization in cultured rat osteoblasts.
Osteoblasts from craniums of newly born SD rats were cultured in vitro. MTT, PNPP and ARS were used to observe the proliferation, activity of ALP and the number of mineral node of cultured osteoblasts in vitro.
It was found that puerarin had the effect on stimulating cell proliferation, activity of ALP and the number of mineral node of cultured osteoblasts (P < 0.01 or P < 0.05).
Puerarin can promote proliferation, differentiation, maturation and mineralization of the osteoblasts in vitro.
观察葛根素对体外培养的大鼠成骨细胞增殖、分化、成熟及矿化的影响。
取新生SD大鼠颅骨成骨细胞进行体外培养，采用MTT法、PNPP法及ARS法分别观察体外培养成骨细胞的增殖、碱性磷酸酶（ALP）活性及矿结节数量。
发现葛根素对体外培养成骨细胞的增殖、ALP活性及矿结节数量有促进作用（P<0.01或P<0.05）。
葛根素可促进体外培养成骨细胞的增殖、分化、成熟及矿化。
