轮状病毒特异性B细胞的特征及其与血清学记忆的关系。
Characterization of rotavirus specific B cells and their relation with serological memory.
作者信息
Rojas Olga Lucía, Narváez Carlos Fernando, Greenberg Harry B, Angel Juana, Franco Manuel A
机构信息
Instituto de Genética Humana, Pontificia Universidad Javeriana, Carrera 7 # 40-62, Bogotá, Colombia.
出版信息
Virology. 2008 Oct 25;380(2):234-42. doi: 10.1016/j.virol.2008.08.004. Epub 2008 Sep 11.
We quantified circulating total, rotavirus (RV) and Tetanus toxin (TT) memory B cells (mBc) in healthy adults using a limiting dilution assay (LDA) and a flow cytometry assay (FCA) that permit evaluation of both CD27+ and CD27- mBc. RV mBc were enriched in the CD27-, IgG+ and in the CD27+, IgM+ subsets. The numbers of RV mBc were higher by FCA than by LDA and results of the two assays did not correlate. TT IgGmBc and RV IgA mBc determined by FCA and by LDA correlated with TT plasma IgG and RV plasma IgA, respectively. The mean ratio of specific mBc/mug/ml of the corresponding plasma immunoglobulin was lower for TT IgG than for RV IgA mBc. Our studies contribute to understand the relationship between circulating mBc and serological memory, and enhance our capacity to develop better correlates of protection against RV disease.
我们使用极限稀释分析（LDA）和流式细胞术分析（FCA）对健康成年人循环中的总记忆B细胞（mBc）、轮状病毒（RV）特异性记忆B细胞和破伤风毒素（TT）特异性记忆B细胞进行了定量分析，这两种分析方法能够对CD27+和CD27-记忆B细胞进行评估。RV特异性记忆B细胞在CD27-、IgG+亚群以及CD27+、IgM+亚群中富集。通过FCA检测到的RV特异性记忆B细胞数量高于LDA检测结果，且两种检测方法的结果不相关。通过FCA和LDA检测确定的TT IgG特异性记忆B细胞和RV IgA特异性记忆B细胞分别与TT血浆IgG和RV血浆IgA相关。TT IgG特异性记忆B细胞与相应血浆免疫球蛋白的每微克/毫升特异性记忆B细胞的平均比值低于RV IgA特异性记忆B细胞。我们的研究有助于理解循环记忆B细胞与血清学记忆之间的关系，并提高我们开发针对RV疾病更好的保护相关性指标的能力。
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