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短期免疫球蛋白A B细胞记忆存在于肠道淋巴组织中,而非接种了Wa人轮状病毒的无菌猪的骨髓中。

Short-term immunoglobulin A B-cell memory resides in intestinal lymphoid tissues but not in bone marrow of gnotobiotic pigs inoculated with Wa human rotavirus.

作者信息

Yuan L, Geyer A, Saif L J

机构信息

Food Animal Health Research Program, Ohio Agricultural Research and Development Center, Department of Veterinary Preventive Medicine, The Ohio State University, Wooster, OH, USA.

出版信息

Immunology. 2001 Jun;103(2):188-98. doi: 10.1046/j.1365-2567.2001.01229.x.

Abstract

Immunological memory is important for protecting the host from reinfection. To investigate the development and sites of residence of intestinal memory B cells, and their role in protective immunity to reinfection with an enteric virus, we assessed the association between memory B cell and antibody-secreting cell (ASC) responses and protection using a gnotobiotic pig model for human rotavirus (HRV) infection and diarrhoea. The isotypes, quantities and tissue distribution of rotavirus-specific memory B cells and ASC were evaluated prechallenge (28 and 83 postinoculation days [PID]) and postchallenge (7 postchallenge days [PCD]), using enzyme-linked immunospot (ELISPOT) assay, in gnotobiotic pigs inoculated once with virulent or three times with attenuated HRV and challenged at PID 28 with the corresponding virulent HRV. Complete protection against HRV shedding and diarrhoea was associated with significantly higher numbers of immunoglobulin A (IgA) and immunoglobulin G (IgG) memory B cells and ASC in the ileum of virulent HRV-inoculated pigs at challenge. In contrast, pigs inoculated with attenuated HRV had lower numbers of IgA and IgG memory B cells and ASC in intestinal lymphoid tissues, but higher numbers in the spleen. The bone marrow had the lowest mean numbers of IgA and IgG memory B cells and ASC prechallenge in both groups of HRV-inoculated pigs. Therefore, bone marrow was not a site for IgA and IgG rotavirus-specific antibody production or for memory B cells after inoculation with live rotavirus, from 28 PID up to at least 83 PID. The effect of in vitro antigen dose was examined and it was determined to play an important role in the development of ASC from memory B cells for the different tissues examined.

摘要

免疫记忆对于保护宿主免受再次感染至关重要。为了研究肠道记忆B细胞的发育、驻留部位及其在抵抗肠道病毒再次感染的保护性免疫中的作用,我们使用了一种用于人类轮状病毒(HRV)感染和腹泻的无菌猪模型,评估了记忆B细胞和抗体分泌细胞(ASC)反应与保护之间的关联。在无菌猪接种一次强毒HRV或三次减毒HRV并在接种后第28天(PID)用相应的强毒HRV攻击之前(接种后28天和83天)和攻击之后(攻击后7天[PCD]),使用酶联免疫斑点(ELISPOT)试验评估轮状病毒特异性记忆B细胞和ASC的亚型、数量及组织分布。在攻击时,对强毒HRV接种猪的回肠中免疫球蛋白A(IgA)和免疫球蛋白G(IgG)记忆B细胞及ASC数量显著增多与对HRV排毒和腹泻的完全保护相关。相比之下,接种减毒HRV的猪在肠道淋巴组织中的IgA和IgG记忆B细胞及ASC数量较少,但在脾脏中的数量较多。在两组接种HRV的猪中,骨髓在攻击前IgA和IgG记忆B细胞及ASC的平均数量最低。因此,从接种后28天直至至少83天,接种活轮状病毒后,骨髓不是IgA和IgG轮状病毒特异性抗体产生的部位,也不是记忆B细胞的驻留部位。我们还研究了体外抗原剂量的影响,结果确定其在不同检测组织中记忆B细胞向ASC的发育过程中起重要作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/84ac/1783226/377c9b44f405/imm0103-0188-f1.jpg

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