Varayoud Jorgelina, Monje Lucas, Bernhardt Tania, Muñoz-de-Toro Mónica, Luque Enrique H, Ramos Jorge G
Laboratorio de Endocrinología y Tumores Hormonodependientes, School of Biochemistry and Biological Sciences, Universidad Nacional del Litoral, Santa Fe, Argentina.
Reprod Toxicol. 2008 Oct;26(2):138-45. doi: 10.1016/j.reprotox.2008.08.004. Epub 2008 Aug 23.
The estrogenic activity of environmentally relevant doses of endosulfan was investigated using an animal model. Ovariectomized adult rats were injected once a day for 3 days with sesame oil (control), 0.02mg/kg/day 17beta-estradiol (an uterotrophic dose; UE(2)), 0.0002mg/kg/day 17beta-estradiol (a non-uterotrophic dose; NUE(2)), or 0.006, 0.06, 0.6 or 6mg/kg/day endosulfan. After 24h of treatment, the uteri were weighed (uterotrophic assay) and the luminal epithelial cell height (LECH) and progesterone receptor (PR), and estrogen receptor alpha (ERalpha) protein levels were measured. PR, ERalpha, and complement factor-3 (C3) mRNAs were evaluated using real-time PCR. Uterine weight and LECH were only increased in UE(2)-treated rats. PR, ERalpha and C3 expression levels were modified in most of the endosulfan-treated groups, showing an identical pattern of expression to the NUE(2)-group. Our results show that the pesticide endosulfan mimics non-uterotrophic E(2) actions, strengthening the hypothesis that endosulfan is a widespread xenoestrogen.
利用动物模型研究了环境相关剂量硫丹的雌激素活性。对成年去卵巢大鼠每天注射一次，持续3天，注射的物质分别为芝麻油（对照组）、0.02mg/kg/天的17β-雌二醇（一种能使子宫增重的剂量；UE(2)）、0.0002mg/kg/天的17β-雌二醇（一种不能使子宫增重的剂量；NUE(2)），或0.006、0.06、0.6或6mg/kg/天的硫丹。治疗24小时后，称量子宫重量（子宫增重试验），并测量管腔上皮细胞高度（LECH）、孕酮受体（PR）以及雌激素受体α（ERα）的蛋白水平。使用实时聚合酶链反应评估PR、ERα和补体因子3（C3）的信使核糖核酸。只有接受UE(2)治疗的大鼠子宫重量和LECH增加。在大多数接受硫丹治疗的组中，PR、ERα和C3的表达水平发生了改变，呈现出与NUE(2)组相同的表达模式。我们的结果表明，农药硫丹模拟了非子宫增重的E(2)作用，强化了硫丹是一种广泛存在的外源性雌激素的假说。
