Baeck Gun-Wook, Kim Joo-Won, Park Chan-Il
Department of Marine Biology and Aquaculture, Institute of Marine Industry, College of Marine Science, Gyeongsang National University, 455 Tongyeong, 650-160 Gyeongnam, South Korea.
Fish Shellfish Immunol. 2008 Nov;25(5):679-81. doi: 10.1016/j.fsi.2008.08.005. Epub 2008 Aug 26.
The interferon stimulated gene 15 (ISG15) is strongly induced in many cell types by IFNs, viral infection and double-stranded RNA (poly I:C). The ISG15 homolog cDNA was isolated from the black rockfish poly I:C stimulated leukocyte cDNA library. The black rockfish ISG15 homolog was found to consist of 1070bp encoding 160 amino acid residues. Compared with other known ISG15 peptide sequences, the most conserved regions of the black rockfish ISG15 peptide were found to be the tandem ubiquitin-like domains and a C-terminal LRLRGG conjugating motif, characteristic of mammalian and non-mammalian ISG15 proteins. A phylogenetic analysis based on the deduced amino acid sequence revealed a homologous relationship between the ISG15 sequence of black rockfish and that of Atlantic salmon, Atlantic cod, crucian carp and rainbow trout. The expression of the black rockfish ISG15 molecule was induced in the peripheral blood leukocytes (PBLs) from 1 to 12h following poly I:C stimulation, with a peak at 6h post-stimulation. The black rockfish gene was predominantly expressed in the PBLs and the spleen.
干扰素刺激基因15（ISG15）在许多细胞类型中被干扰素、病毒感染和双链RNA（聚肌胞苷酸）强烈诱导。ISG15同源cDNA从黑鲪聚肌胞苷酸刺激的白细胞cDNA文库中分离出来。发现黑鲪ISG15同源物由1070bp组成，编码160个氨基酸残基。与其他已知的ISG15肽序列相比，发现黑鲪ISG15肽最保守的区域是串联泛素样结构域和C端LRLRGG缀合基序，这是哺乳动物和非哺乳动物ISG15蛋白的特征。基于推导氨基酸序列的系统发育分析揭示了黑鲪的ISG15序列与大西洋鲑、大西洋鳕鱼、鲫鱼和虹鳟的ISG15序列之间的同源关系。聚肌胞苷酸刺激后1至12小时，黑鲪ISG15分子在外周血白细胞（PBL）中表达，刺激后6小时达到峰值。黑鲪基因主要在PBL和脾脏中表达。
