Zhang S, Zhu C, Wu Y, Zhai W, Shi J, Song Y, Li J, Li Z, Zhang G
The First Affiliated Hospital of Zhengzhou University, Zhengzhou, China.
Transplant Proc. 2008 Sep;40(7):2159-62. doi: 10.1016/j.transproceed.2008.06.053.
The purpose of this study was to observe how the brain-death state affects the hepatic immunogenicity of pigs.
Twelve healthy ChangBai pigs were allocated randomly to 2 groups: control group (n = 6) with Foley catheter balloon placed intracalvarium for 24 hours only versus brain-death group (n = 6) with brain death for 24 hours. The serum and hepatic tissues were obtained at 6, 12, and 24 hours after the initial confirmation of brain death. Tumor necrosis factor (TNF)-alpha and interleukin (IL)-6 were determined by enzyme-linked immunosorbent assay (ELISA). The major histocompatibility complex class II (MHC-II) was determined by immunohistochemistry, and the intercellular adhesion molecule-1 (ICAM-1) mRNA and monocyte chemotactic protein-1 (MCP-1) mRNA by real-time polymerase chain reaction (PCR).
The serum TNF-alpha and IL-6, MHC-II, ICAM-1 mRNA, and MCP-1 mRNA in hepatic tissues in the brain-death group were higher than in the control group; they all increased with time.
Brain death increased liver immunogenicity, which may increase the immune lesion and rejection after transplantation.
本研究旨在观察脑死亡状态如何影响猪的肝脏免疫原性。
将12只健康的长白猪随机分为2组：对照组（n = 6），仅在颅腔内放置Foley导尿管球囊24小时；脑死亡组（n = 6），脑死亡24小时。在首次确认脑死亡后的6小时、12小时和24小时采集血清和肝脏组织。采用酶联免疫吸附测定（ELISA）法测定肿瘤坏死因子（TNF）-α和白细胞介素（IL）-6。通过免疫组织化学法测定主要组织相容性复合体II类（MHC-II），通过实时聚合酶链反应（PCR）测定细胞间黏附分子-1（ICAM-1）mRNA和单核细胞趋化蛋白-1（MCP-1）mRNA。
脑死亡组肝脏组织中的血清TNF-α、IL-6、MHC-II、ICAM-1 mRNA和MCP-1 mRNA均高于对照组；它们均随时间增加。
脑死亡增加了肝脏免疫原性，这可能会增加移植后的免疫损伤和排斥反应。
