Li Zongfang, Zhang Shu, Huang Chen, Zhang Wei, Hu Yajun, Wei Bo
The Second Affiliated Hospital, School of Medicine, Xi'an Jiaotong University, Xi'an, Shaanxi province, China.
Exp Biol Med (Maywood). 2008 Nov;233(11):1454-61. doi: 10.3181/0711-RM-321. Epub 2008 Sep 12.
MicroRNAs (miRNAs) are a recently discovered class of post-transcriptional regulators of gene expression with critical functions in health and disease. Their role in the pathogenesis of hypersplenism, however, is completely unknown. To determine whether miRNA expression is altered in splenic macrophages associated with hypersplenism due to portal hypertension in hepatitis-B-virus (HBV)-related cirrhosis, we analyzed the entire miRNAome in macrophages from normal and portal hypertensive spleen samples by microarray and Real-Time PCR. In this study, we identified 99 miRNA differences in expression in splenic macrophages associated with hypersplenism due to portal hypertension in HBV-related cirrhosis. Among the miRNAs identified in this study, hsa-miR-615-3p was significantly up-regulated in hypersplenism. Dynamic changes in miRNA expression occurred during the pathogenesis of portal hypertension-induced hypersplenism in HBV-related cirrhosis. The miRNAs then are novel regulatory RNAs in hypersplenism in patients with HBV-related cirrhosis.
微小RNA（miRNA）是最近发现的一类基因表达的转录后调节因子，在健康和疾病中发挥着关键作用。然而，它们在脾功能亢进发病机制中的作用完全未知。为了确定与乙型肝炎病毒（HBV）相关肝硬化门静脉高压所致脾功能亢进相关的脾巨噬细胞中miRNA表达是否发生改变，我们通过微阵列和实时PCR分析了正常和门静脉高压脾脏样本中巨噬细胞的整个miRNA组。在本研究中，我们在与HBV相关肝硬化门静脉高压所致脾功能亢进相关的脾巨噬细胞中鉴定出99种miRNA表达差异。在本研究中鉴定出的miRNA中，hsa-miR-615-3p在脾功能亢进中显著上调。在HBV相关肝硬化门静脉高压诱导的脾功能亢进发病过程中，miRNA表达发生动态变化。这些miRNA是HBV相关肝硬化患者脾功能亢进中的新型调节RNA。
