Eastman S, Markholst H, Wilson D, Lernmark A
Department of Medicine, University of Washington, Seattle 98195.
Diabetes Res Clin Pract. 1991 May;12(2):113-23. doi: 10.1016/0168-8227(91)90088-u.
Inbred lymphopenic, diabetes-prone (DP) and non-lymphopenic, diabetes-resistant (DR) BB rats in a specific pathogen-free (SPF) colony were subjected to a cross-intercross breeding experiment which showed diabetes to segregate as a recessive trait. All DP rats, but none of the DR and F1 rats, developed diabetes. In contrast, about 25% of the F2 rats developed diabetes which made it possible to study these rats without maternal influence of diabetes. All rats were bled at regular intervals between 30 and 150 days of age, and the samples analyzed for numbers of leukocytes, lymphocytes, neutrophils, monocytes and eosinophils. Leukocyte numbers tended to increase with age until about 100 days, and to decline thereafter. Males had more leukocytes than females. Coinciding with the time of onset of overt diabetes, there was a large increase in eosinophils, along with smaller increases in neutrophils, monocytes and lymphocytes. These data in SPF DP and DR BB rats and their cross-intercross offspring demonstrate that the overt onset of diabetes is associated with a significant leukocytosis.
在一个无特定病原体（SPF）的种群中，对近交系淋巴细胞减少、易患糖尿病（DP）和非淋巴细胞减少、抗糖尿病（DR）的BB大鼠进行了回交-互交繁殖实验，结果显示糖尿病作为隐性性状分离。所有DP大鼠都患上了糖尿病，但DR大鼠和F1大鼠均未患病。相比之下，约25%的F2大鼠患上了糖尿病，这使得在没有糖尿病母体影响的情况下研究这些大鼠成为可能。所有大鼠在30至150日龄期间定期采血，对样本中的白细胞、淋巴细胞、中性粒细胞、单核细胞和嗜酸性粒细胞数量进行分析。白细胞数量在约100日龄前趋于随年龄增加，之后则下降。雄性大鼠的白细胞比雌性大鼠多。与明显糖尿病发病时间一致，嗜酸性粒细胞大幅增加，同时中性粒细胞、单核细胞和淋巴细胞也有较小幅度的增加。这些在SPF DP和DR BB大鼠及其回交-互交后代中的数据表明，糖尿病的明显发病与显著的白细胞增多有关。
