人类淋巴细胞中的DNA链断裂代谢：重新评估

DNA strand break metabolism in human lymphocytes: a reevaluation.

作者信息

Boerrigter M E

机构信息

Harvard Medical School, Beth Israel Hospital, Gerontology Division, Boston, Massachusetts 02215.

出版信息

Exp Cell Res. 1991 Sep;196(1):1-5. doi: 10.1016/0014-4827(91)90448-4.

DOI:10.1016/0014-4827(91)90448-4

PMID:1879462

Abstract

Recent findings concerning the presumed existence of single-strand breaks (SSB) in quiescent human peripheral blood lymphocytes (PBL) are discussed in relation to the role of poly(ADP-ribosyl)ation in DNA strand break metabolism. It is argued that the activation of poly(ADP-ribose)polymerase (ADPRP) by a DNA-damaging agent is not indicative of an obligatory role of poly(ADP-ribosyl)ation in DNA repair. From this it follows that SSB induced by different strand-breaking agents might be removed by either ADPRP-dependent or ADPRP-independent DNA repair pathways.

摘要

本文讨论了关于静止期人外周血淋巴细胞（PBL）中可能存在单链断裂（SSB）的最新研究结果，并探讨了聚（ADP-核糖基）化在DNA链断裂代谢中的作用。有人认为，DNA损伤剂激活聚（ADP-核糖）聚合酶（ADPRP）并不意味着聚（ADP-核糖基）化在DNA修复中起必然作用。由此可见，不同的链断裂剂诱导产生的SSB可能通过依赖ADPRP或不依赖ADPRP的DNA修复途径来修复。