Michishita M, Hirayoshi K, Tsuru A, Nakamura N, Yoshida Y, Okuma M, Nagata K
Division of Internal Medicine, Faculty of Medicine, Kyoto University, Japan.
Exp Cell Res. 1991 Sep;196(1):107-13. doi: 10.1016/0014-4827(91)90461-3.
Murine myelomonocytic leukemia M1 cells have been used to examine the effects of type-beta 1 transforming growth factor (TGF-beta 1) on cellular proliferation and differentiation in monocyte-macrophage lineage. TGF-beta 1 inhibited immature M1 cell growth due to a general slowdown of the cell cycle, without arrest at any specific point. Ten nanograms per milliliter TGF-beta 1 completely suppressed phagocytic activity and adhesion to the dish surface and partially inhibited the expression of Fc receptors and vimentin during the differentiation of M1 cells induced by IL-6. IL-6-induced declines in the expression of c-myc mRNA and in the accumulation of G0/G1 cells were also partially blocked by TGF-beta 1. When treated concurrently with IL-6 and TGF-beta 1, approximately 50% of M1 cells were morphologically converted to promonocyte or monocyte-like cells, which did not exhibit the characteristics of mature macrophages. Although pretreatment with TGF-beta 1 also inhibited the IL-6-induced phagocytic activity, this inhibition was reversible. Once TGF-beta 1 was removed from the culture medium after 72 h of incubation with IL-6, the kinetics of differentiation induced by IL-6 were faster in pretreated cells than in nonpretreated cells. TGF-beta 1 appears to inhibit the IL-6 induced conversion of M1 cells at the intermediate stage of monocytic differentiation.
小鼠骨髓单核细胞白血病M1细胞已被用于研究β1型转化生长因子（TGF-β1）对单核细胞-巨噬细胞谱系细胞增殖和分化的影响。TGF-β1抑制未成熟M1细胞的生长，这是由于细胞周期普遍减慢，而非停滞于任何特定阶段。每毫升10纳克的TGF-β1完全抑制吞噬活性和对培养皿表面的黏附，并在IL-6诱导M1细胞分化过程中部分抑制Fc受体和波形蛋白的表达。TGF-β1还部分阻断了IL-6诱导的c-myc mRNA表达下降和G0/G1期细胞的积累。当与IL-6和TGF-β1同时处理时，约50%的M1细胞在形态上转化为前单核细胞或单核细胞样细胞，这些细胞不表现出成熟巨噬细胞的特征。尽管用TGF-β1预处理也会抑制IL-6诱导的吞噬活性，但这种抑制是可逆的。在用IL-6孵育72小时后，一旦从培养基中去除TGF-β1，预处理细胞中由IL-6诱导的分化动力学比未预处理细胞更快。TGF-β1似乎在单核细胞分化的中间阶段抑制IL-6诱导的M1细胞转化。
