Miyaura C, Onozaki K, Akiyama Y, Taniyama T, Hirano T, Kishimoto T, Suda T
Department of Biochemistry, School of Dentistry, Showa University, Tokyo, Japan.
FEBS Lett. 1988 Jul 4;234(1):17-21. doi: 10.1016/0014-5793(88)81293-6.
Recombinant human interleukin 6 (IL-6), a lymphokine involved in the final differentiation of activated B-cells into antibody-forming cells, greatly suppressed proliferation and induced differentiation of murine myeloid leukemia cells (M1) into mature macrophage-like cells. When M1 cells were treated with IL-6, their growth was completely arrested as early as on day 2, and they were induced to differentiate morphologically into macrophage-like cells. Differentiation-associated properties such as phagocytic activity, adherence to the dish surface, Fc and C3 receptors, were also induced within 24 h by IL-6, and they reached their respective maximal levels on day 2 or 3. The potency of IL-6 in suppressing proliferation and inducing differentiation was much greater than that of 1 alpha,25-dihydroxyvitamin D3 one of the most potent inducers of M1 cells. The present report indicates that IL-6 is involved in the differentiation of not only B-cells but also myeloid leukemia cells.
重组人白细胞介素6（IL-6）是一种参与激活的B细胞最终分化为抗体形成细胞的淋巴因子，它能极大地抑制小鼠髓系白血病细胞（M1）的增殖，并诱导其分化为成熟的巨噬细胞样细胞。当用IL-6处理M1细胞时，其生长早在第2天就完全停止，并且它们在形态上被诱导分化为巨噬细胞样细胞。IL-6还能在24小时内诱导出与分化相关的特性，如吞噬活性、对培养皿表面的粘附、Fc和C3受体，并且它们在第2天或第3天达到各自的最高水平。IL-6在抑制增殖和诱导分化方面的效力远大于1α,25-二羟基维生素D3，后者是M1细胞最有效的诱导剂之一。本报告表明，IL-6不仅参与B细胞的分化，也参与髓系白血病细胞的分化。
