Depan Dilip, Kumar Annamalai Pratheep, Singh Raj Pal
Division of Polymer Science and Engineering, National Chemical Laboratory, Dr. Homi Bhabha Road, Pune, Maharashtra 411 008, India.
Acta Biomater. 2009 Jan;5(1):93-100. doi: 10.1016/j.actbio.2008.08.007. Epub 2008 Aug 29.
The present paper reveals the potential uses of novel hybrids of chitosan-g-lactic acid and sodium montmorillonite (MMT) in controlled drug delivery and tissue engineering applications. The drug-loaded novel nanohybrid films and porous scaffolds have been prepared by solvent casting and freeze-drying of the grafted polymer solution, respectively. Sodium Ibuprofen was loaded into nanohybrids of chitosan-g-lactic acid/sodium montmorillonite (CS-g-LA/MMT). Grafting of lactic acid and the drug loading were characterized by Fourier transform infrared spectroscopy. Formation of intercalated nanocomposites was confirmed by X-ray diffraction. Mechanical properties measurements have shown improvement in modulus and strength with expense of elongation by MMT reinforcement. The nanohybrids were found to be stable regardless of pH of the medium. The cell proliferation profile also shows that prepared nanohybrids are biocompatible. MMT reinforcement was found to control the drug (Ibuprofen) release rate in phosphate buffer saline solution (pH 7.4). MMT clay is therefore a viable additive for formulating sustained drug delivery systems based on lactic acid grafted chitosan.
本文揭示了壳聚糖 - g - 乳酸与钠基蒙脱石（MMT）的新型杂化物在控释药物递送和组织工程应用中的潜在用途。分别通过接枝聚合物溶液的溶剂浇铸和冷冻干燥制备了载药新型纳米杂化薄膜和多孔支架。将布洛芬钠载入壳聚糖 - g - 乳酸/钠基蒙脱石（CS - g - LA/MMT）的纳米杂化物中。通过傅里叶变换红外光谱对乳酸接枝和药物载入进行了表征。通过X射线衍射证实了插层纳米复合材料的形成。力学性能测量表明，通过MMT增强，模量和强度有所提高，但伸长率有所降低。发现纳米杂化物无论介质的pH值如何都很稳定。细胞增殖情况还表明所制备的纳米杂化物具有生物相容性。发现MMT增强可控制药物（布洛芬）在磷酸盐缓冲盐水溶液（pH 7.4）中的释放速率。因此，MMT粘土是用于配制基于乳酸接枝壳聚糖的持续药物递送系统的可行添加剂。
