短柄棒杆菌胆固醇氧化酶编码基因choB的序列：与链霉菌SA-COO的choA比较。

Sequence of gene choB encoding cholesterol oxidase of Brevibacterium sterolicum: comparison with choA of streptomyces sp. SA-COO.

作者信息

Ohta T, Fujishiro K, Yamaguchi K, Tamura Y, Aisaka K, Uwajima T, Hasegawa M

机构信息

Tokyo Research Laboratories, Kyowa Hakko Kogyo Co., Ltd., Japan.

出版信息

Gene. 1991 Jul 15;103(1):93-6. doi: 10.1016/0378-1119(91)90397-t.

DOI:10.1016/0378-1119(91)90397-t

PMID:1879703

Abstract

The nucleotide (nt) sequence of the cholesterol oxidase (Cho)-encoding gene (choB) cloned from Brevibacterium sterolicum ATCC21387 was determined. The sequence contained an open reading frame with a G + C content of 64.9 mol% that would encode a protein of 552 amino acids (aa). Comparison of the nt sequence of choB and deduced aa sequence to those of the Cho-encoding gene (choA) of Streptomyces sp. strain SA-COO showed identities of 64% and 58%, respectively. N-terminal aa sequence analysis of the extracellular enzyme of B. sterolicum confirmed that the mature enzyme consisted of 507 aa with a predicted Mr of 54,902, and was preceded by a 45-aa signal sequence.

摘要

测定了从短柄棒杆菌ATCC21387克隆的胆固醇氧化酶（Cho）编码基因（choB）的核苷酸（nt）序列。该序列包含一个开放阅读框，G+C含量为64.9 mol%，可编码一个由552个氨基酸（aa）组成的蛋白质。将choB的nt序列和推导的aa序列与链霉菌属菌株SA - COO的Cho编码基因（choA）的相应序列进行比较，结果显示同源性分别为64%和58%。对短柄棒杆菌细胞外酶的N端aa序列分析证实，成熟酶由507个aa组成，预测的Mr为54,902，其前面有一个45个aa的信号序列。