D3受体基因敲除小鼠脊髓中未改变的D1、D2、D4和D5多巴胺受体mRNA表达及分布
Unaltered D1, D2, D4, and D5 dopamine receptor mRNA expression and distribution in the spinal cord of the D3 receptor knockout mouse.
作者信息
Zhu Hong, Clemens Stefan, Sawchuk Michael, Hochman Shawn
机构信息
Department of Physiology, Emory University School of Medicine, Atlanta, GA 30322, USA.
出版信息
J Comp Physiol A Neuroethol Sens Neural Behav Physiol. 2008 Nov;194(11):957-62. doi: 10.1007/s00359-008-0368-5. Epub 2008 Sep 17.
Abstract
Dopamine (DA) acts through five receptor subtypes (D1-D5). We compared expression levels and distribution patterns of all DA mRNA receptors in the spinal cord of wild-type (WT) and loss of function D3 receptor knockout (D3KO) animals. D3 mRNA expression was increased in D3KO, but no D3 receptor protein was associated with cell membranes, supporting the previously reported lack of function. In contrast, mRNA expression levels and distribution patterns of D1, D2, D4, and D5 receptors were similar between WT and D3KO animals. We conclude that D3KO spinal neurons do not compensate for the loss of function of the D3 receptor with changes in the other DA receptor subtypes. This supports use of D3KO animals as a model to provide insight into D3 receptor dysfunction in the spinal cord.
摘要
多巴胺（DA）通过五种受体亚型（D1 - D5）发挥作用。我们比较了野生型（WT）和功能缺失的D3受体敲除（D3KO）动物脊髓中所有DA mRNA受体的表达水平和分布模式。D3KO中D3 mRNA表达增加，但没有D3受体蛋白与细胞膜相关联，这支持了先前报道的功能缺失。相比之下，WT和D3KO动物之间D1、D2、D4和D5受体的mRNA表达水平和分布模式相似。我们得出结论，D3KO脊髓神经元不会通过其他DA受体亚型的变化来补偿D3受体功能的丧失。这支持将D3KO动物用作模型，以深入了解脊髓中D3受体功能障碍。
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