Zhang Xinwei, Wang Min, Zhou Chunlin, Chen Sen, Wang Jianxiang
Department of Clinical Hematology, Institute of Hematology and Blood Diseases Hospital, Chinese Academy of Medical Sciences and Peking Union Medical College, 288 Nanjing Road, Tianjin 300020, P.R.China.
Leuk Res. 2005 Feb;29(2):179-83. doi: 10.1016/j.leukres.2004.07.001.
Inhibitory member of the ASPP family (iASPP) is an evolutionarily conserved inhibitor of p53, and its expression is upregulated in human breast carcinomas expressing wild-type p53. To examine the role of iASPP in acute leukemia (AL), we analyzed iASPP mRNA expression in AL by semi-quantitative reverse transcriptase polymerase chain reaction (RT-PCR). PCR products were confirmed by restriction endonuclease BstX I digestion and sequencing analysis. The results showed that median levels of iASPP gene expression in cells of AL were significantly higher than those in cells from normal donors and AL patients in complete remission (CR) (P = 0.019, 0.021, respectively). There was no significant difference between acute lymphocytic leukemia (ALL) cells and acute myeloid leukemia (AML) cells (P = 0.593). The expression level of iASPP gene and its overexpression in M3 and M4EO were significantly lower than in other subtypes of AML. However, iASPP gene expression in AL cells was not associated with gender, age, initial white blood cell count or p53 type, but was associated with CD34 expression. The results of the present study suggest that iASPP gene overexpression may play an important role in the leukemogenesis and/or disease progression of AL.
ASPP家族的抑制成员（iASPP）是一种在进化上保守的p53抑制剂，其在表达野生型p53的人类乳腺癌中表达上调。为了研究iASPP在急性白血病（AL）中的作用，我们通过半定量逆转录聚合酶链反应（RT-PCR）分析了AL中iASPP mRNA的表达。PCR产物通过限制性内切酶BstX I消化和测序分析进行确认。结果显示，AL细胞中iASPP基因表达的中位数水平显著高于正常供体和完全缓解（CR）的AL患者细胞中的水平（分别为P = 0.019，0.021）。急性淋巴细胞白血病（ALL）细胞和急性髓细胞白血病（AML）细胞之间无显著差异（P = 0.593）。iASPP基因在M3和M4EO中的表达水平及其过表达显著低于其他AML亚型。然而，AL细胞中iASPP基因的表达与性别、年龄、初始白细胞计数或p53类型无关，但与CD34表达有关。本研究结果表明，iASPP基因过表达可能在AL的白血病发生和/或疾病进展中起重要作用。
