Onetti-Muda A, Crescenzi A, Pujia N, Faraggiana T, Marinozzi V
Dipartimento Biopatologia Umana, Università di Roma, La Sapienza, Italy.
Histopathology. 1991 Jun;18(6):511-6. doi: 10.1111/j.1365-2559.1991.tb01477.x.
Immunohistochemical evaluation of oestrogen and progesterone receptors is of importance in evaluating human breast tumours. Staining techniques can be performed on snap-frozen, cryostat-cut tissues or, as recently reported, on formalin-fixed, paraffin-embedded tissues. These methods are, however, limited by several drawbacks, including difficulties in retrospective studies and in storage of the material, and the relatively high frequency of false negative results for chemically fixed specimens. We therefore investigated the application of freeze-drying technology to assess the feasibility and reliability of this technique as an alternative method for diagnostic breast pathology. Morphological and immunohistochemical studies were performed on snap-frozen, freeze-dried and paraffin-embedded tissue obtained from 16 cases of benign and malignant breast neoplasms. Our results showed good preservation of tissue morphology, similar to standard formalin fixation, and excellent preservation of antigenic reactivity of nuclear receptors, comparable to that obtained with cryostat sections. We therefore suggest that freeze drying and paraffin embedding of frozen tissue blocks is equivalent or even preferable to formalin fixation for the demonstration of oestrogen and progesterone receptors, at least in the case of small tumours.
雌激素和孕激素受体的免疫组织化学评估在评估人类乳腺肿瘤中具有重要意义。染色技术可在速冻、低温恒冷切片的组织上进行，或者如最近报道的那样，在福尔马林固定、石蜡包埋的组织上进行。然而，这些方法存在几个缺点，包括回顾性研究和材料储存方面的困难，以及化学固定标本假阴性结果的相对高发生率。因此，我们研究了冷冻干燥技术的应用，以评估该技术作为诊断乳腺病理学替代方法的可行性和可靠性。对取自16例乳腺良恶性肿瘤的速冻、冷冻干燥和石蜡包埋组织进行了形态学和免疫组织化学研究。我们的结果显示，组织形态保存良好，类似于标准福尔马林固定，核受体的抗原反应性保存优异，与低温恒冷切片相当。因此，我们建议，至少对于小肿瘤，冷冻组织块的冷冻干燥和石蜡包埋在显示雌激素和孕激素受体方面等同于甚至优于福尔马林固定。
