子宫内膜异位症组织中雌激素和孕激素受体的免疫组织化学检测。石蜡包埋组织与新鲜冷冻组织的比较研究。
Immunohistochemical detection of estrogen and progesterone receptors in endometriotic tissue. A comparative study of paraffin embedded and fresh frozen tissues.
作者信息
Regidor P A, Regidor M, Metz K A, Schindler A E
机构信息
Abteilung für Gynäkologie, insbesondere gynäkologische Onkologie, Zentrum für Frauenheilkunde, Universitätsklinik Essen, Germany.
出版信息
Arch Gynecol Obstet. 1994;255(4):181-7. doi: 10.1007/BF02335083.
30 formalin-fixed and paraffin embedded and 20 fresh frozen samples of endometriotic tissue were analysed immunohistochemically for the concentration of estrogen and progesterone receptors. In the formalin-fixed and paraffin embedded group only 37% of the samples were estrogen receptor positive whereas 63% were receptor negative. In contrast, we found that 67% of the samples had a positive progesterone receptor status. In the fresh frozen group 60% of endometriotic tissues were estrogen receptor positive and 75% of the tissues had a positive progesterone receptor status. We could not find any correlation between the site or severity of the endometriosis or the hormonal receptor status. We were able to demonstrate that the immunohistochemical detection of hormonal receptors in endometriotic tissues is possible and that better results were obtained if fresh frozen rather than formalin-fixed and paraffin embedded tissues were analyzed.
对30份福尔马林固定石蜡包埋的子宫内膜异位组织样本和20份新鲜冰冻样本进行免疫组织化学分析，以检测雌激素和孕激素受体的浓度。在福尔马林固定石蜡包埋组中，仅37%的样本雌激素受体呈阳性，而63%的样本受体呈阴性。相比之下，我们发现67%的样本孕激素受体状态为阳性。在新鲜冰冻组中，60%的子宫内膜异位组织雌激素受体呈阳性，75%的组织孕激素受体状态为阳性。我们未发现子宫内膜异位的部位或严重程度与激素受体状态之间存在任何相关性。我们能够证明，对子宫内膜异位组织中的激素受体进行免疫组织化学检测是可行的，并且如果分析新鲜冰冻组织而非福尔马林固定石蜡包埋组织，能获得更好的结果。
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