Muda A O, Crescenzi A, Mercorella I, Faraggiana T, Marinozzi V
Dipartimento Biopatologia Umana, Università di Roma, La Sapienza, Italy.
Mod Pathol. 1990 Mar;3(2):211-5.
Adequate handling of renal biopsies requires processing for light and electron microscopy, as well as for immunohistochemical study. Problems of adequate sampling are frequently encountered, and tissue size can be insufficient, since morphologic examination requires chemical fixation, while immunofluorescence study is currently performed on snap-frozen, cryostat-cut tissue. The application of freeze-drying technology on 20 renal needle biopsies has been investigated to assess the feasibility and reliability of the technique as a routine diagnostic tool in renal pathology. Morphologic as well as immunohistochemical studies were performed on freeze-dried, paraffin-embedded specimens, including immunofluorescence and PAP techniques to detect immunoglobulins, complement fractions, fibrinogen, collagen types, and laminin. Our results showed good preservation of tissue morphology, similar to standard formalin fixation. Moreover, absence of diffusion artifacts and intensity of immunohistochemical reactions were comparable to what is obtained with cryostat sections. We therefore suggest that freeze-drying before paraffin embedding is, at least for diagnostic purposes, preferable to formalin fixation; unfixed, cryostat-cut sections can still be informative and should be used whenever tissue is available in selected cases, and/or in experimental work.
对肾活检标本进行妥善处理需要进行光镜和电镜检查，以及免疫组化研究。经常会遇到标本采集不充分的问题，而且组织大小可能不足，因为形态学检查需要化学固定，而免疫荧光研究目前是在速冻、经低温恒温器切片的组织上进行的。我们研究了冻干技术在20例肾穿刺活检标本上的应用，以评估该技术作为肾病理学常规诊断工具的可行性和可靠性。对冻干、石蜡包埋的标本进行了形态学和免疫组化研究，包括免疫荧光和PAP技术，以检测免疫球蛋白、补体成分、纤维蛋白原、胶原类型和层粘连蛋白。我们的结果显示组织形态保存良好，与标准福尔马林固定相似。此外，没有扩散假象，免疫组化反应的强度与低温恒温器切片相当。因此，我们建议，至少出于诊断目的，石蜡包埋前的冻干优于福尔马林固定；未固定的低温恒温器切片仍可能提供有用信息，在选定病例的组织可用时，和/或在实验工作中，应随时使用。
