用于研究细胞黏附及活细胞图案化的DNA包被原子力显微镜悬臂
DNA-coated AFM cantilevers for the investigation of cell adhesion and the patterning of live cells.
作者信息
Hsiao Sonny C, Crow Ailey K, Lam Wilbur A, Bertozzi Carolyn R, Fletcher Daniel A, Francis Matthew B
机构信息
Department of Chemistry, University of California, Berkeley.
出版信息
Angew Chem Int Ed Engl. 2008;47(44):8473-7. doi: 10.1002/anie.200802525.
Abstract
Measurement of receptor adhesion strength requires the precise manipulation of single cells on a contact surface. To attach live cells to a moveable probe, DNA sequences complementary to strands displayed on the plasma membrane are introduced onto AFM cantilevers (see picture, bp=base pairs). The strength of the resulting linkages can be tuned by varying the length of DNA strands, allowing for controlled transport of the cells
摘要
测量受体黏附强度需要在接触表面精确操控单个细胞。为了将活细胞附着到可移动探针上，将与质膜上展示的链互补的DNA序列引入原子力显微镜（AFM）悬臂（见图，bp =碱基对）。通过改变DNA链的长度可以调节所得连接的强度，从而实现对细胞的可控运输。
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