Mathis Katelyn, Chan Clement T Y, Meckes Brian
Department of Biomedical Engineering, University of North Texas, 3940 N Elm St., Denton, TX, 76207, USA.
BioDiscovery Institute, University of North Texas, 1155 Union Circle, Denton, TX, 76203 5017, USA.
Adv Healthc Mater. 2024 Dec;13(32):e2402876. doi: 10.1002/adhm.202402876. Epub 2024 Oct 14.
The creation of complex cellular environments is critical to mimicking tissue environments that will play a critical role in next-generation tissue engineering, stem cell programming, and therapeutic screening. To address this growing need, techniques capable of manipulating cell-cell and cell-material interactions are required that span single-cell to 3D tissue architectures. DNA programmed assembly and placement of cells present a powerful technique for the bottom-up synthesis of living microtissues for probing key questions in cell-cell and cell-material-driven behaviors through its refined control over placement and architecture. This review examines the current state of the art in the programming of cellular interactions with DNA and its applications spanning tissue model building, fundamental cellular biology, and cell manipulation for measurements across a host of applications.
创建复杂的细胞环境对于模拟组织环境至关重要，而组织环境在下一代组织工程、干细胞编程和治疗筛选中起着关键作用。为满足这一日益增长的需求，需要能够操纵细胞-细胞和细胞-材料相互作用的技术，这些技术涵盖从单细胞到三维组织结构。DNA编程的细胞组装和定位为自下而上合成活的微组织提供了一种强大的技术，通过对细胞定位和结构的精确控制来探究细胞-细胞和细胞-材料驱动行为中的关键问题。本综述考察了利用DNA进行细胞相互作用编程的当前技术水平及其在组织模型构建、基础细胞生物学以及众多应用中的细胞操纵测量等方面的应用。
