[人MCF-7乳腺癌细胞系不同亚群细胞的体外克隆及体内致瘤能力分析]
[Analysis on clone in vitro and tumorigenic capacity in vivo of different subsets cells from the MCF-7 human breast cancer cell line].
作者信息
Li Zhi, Liu Chun-ping, He Yan-li, Tian Yuan, Huang Tao
机构信息
Department of Breast and Thyroid Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
出版信息
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jul;39(4):547-9.
OBJECTIVE
To investigate whether there are cancer stem cells in the MCF-7 human breast cancer cell line.
METHODS
Flow cytometry was applied to separate different subpopulation cells from MCF-7 cells, and their ability of clone in vitro and reconstruction tumor in vivo were determined.
RESULTS
The ability of clone in vitro and reconstruction tumor in vivo were observed in some MCF-7 cells. Contrast with CD44+ CD24+ cells, the proportion of tumorigenic cancer cells in CD44+ CD24- cells is higher.
CONCLUSION
Breast cancer stem cell exists in MCF-7 and it mainly locates the subpopulation of CD44+ CD24- cells, CD44+ CD24+ cell possibly is breast cancer progenitor cell.
目的
研究MCF-7人乳腺癌细胞系中是否存在癌症干细胞。
方法
应用流式细胞术从MCF-7细胞中分离不同亚群细胞，并测定其体外克隆能力和体内重建肿瘤的能力。
结果
在部分MCF-7细胞中观察到体外克隆能力和体内重建肿瘤的能力。与CD44+CD24+细胞相比，CD44+CD24-细胞中致瘤癌细胞的比例更高。
结论
MCF-7中存在乳腺癌干细胞，其主要定位于CD44+CD24-细胞亚群，CD44+CD24+细胞可能是乳腺癌祖细胞。
Zotero 插件