Mao Wei-dong, Sun Shao-hua
Institute of Pathology, The First Hospital of Lanzhou University, Lanzhou 730000, China.
Sichuan Da Xue Xue Bao Yi Xue Ban. 2008 Jul;39(4):591-4.
To investigate the significance of ulinastatin's effects on kidney's expression of IL-6, HSP70 after mechanical injury.
Sixity-six adult female Wistar rats were randomly divided into three groups: 6 in the group of control, 30 in the group of trauma, 30 in the group injected ulinastatin after trauma. The model of trauma rats was made with free-fall bio-impactor striking the spinal column and ribs of rats. To detect the IL-6 and HSP70 expression of each group's renal tissue at 1 h, 6 h, 12 h, 24 h and 36 h after trauma with tissue chip and immunohistochemical stain.
IL-6 and HSP70 expression of renal tissue was low with group of control. Compared with group of control, the expression of IL-6 strengthened at 1 h, 6 h, 12 h, 24 h after trauma (P < 0.05),the expression of HSP70 with group of trauma strengthened at 6 h after trauma (P < 0. 05). The expression of IL-6 with group injected ulinastatin after trauma were more lower than group of trauma, the expression of HSP70 at 1 h, 24 h, 36 h after trauma was higher than group of trauma especially at 1 h (P< 0.05).
Ulinastatin can reduce the risk of renal injury in early times after trauma especially at 1 h. The protective effects of ulinastatin may be associated with the restrict the expression of IL-6, up-regulation of HSP70 expression, and change the proportion between IL-6 and HSP70 expression.
