热休克蛋白 70 在急性百草枯中毒大鼠肺组织中的表达及乌司他丁的干预作用。
Expression of heat shock protein 70 in lung tissues of acute paraquat poisoned rats and intervention of ulinastatin.
机构信息
Neurosurgery ICU, Banan District People's Hospital, Chongqing 401320, China (Zhang ZJ); Emergemcy Medicine Department, The First Affiliated Hospital of Nanchang University, Nanchang 330006, China (Zhou CY, Luo YJ, Xiong HW).
出版信息
World J Emerg Med. 2010;1(3):229-33.
BACKGROUND
Paraquat (PQ) is an effective herbicide and is widely used in agricultural production, but PQ poisoning is frequently seen in humans with the lung as the target organ. Clinically pulmonary pathological changes are often used to predict the severity and prognosis of the patients. In this study, we observed the expression of heat shock protein 70 (HSP70) in rat lung after PQ poisoning and to investigate the therapeutic effects of ulinastatin.
METHODS
Seventy-two adult healthy SD rats were randomly divided into a control group (group A, n=24), a poisoning group (group B, n=24), and an ulinastatin group (group C, n=24). The rat models of acute PQ poisoning were established by intra-gastric administration of 80 mg/kg PQ to rats of groups B and C, and the rats of group C were intra-peritoneally injected with 100 000 IU/kg ulinastatin 30 minutes after poisoning. The expression of HSP70 in lung tissue was observed, and W/D and histopathological changes in the lung tissue were compared 12, 24, 48 and 72 hours after poisoning. The expression of HSP70 in the lung tissue was assayed by using RT-PCR. All quantitative data were processed with one-way analysis of variance to compare multiple sample means.
RESULTS
Compared to group A, the expression of HSP70 in the lung of rats in groups B and C increased significantly at all intervals (P<0.05). The pathological changes in lung tissue of rats with PQ poisoning included congestion, leukocytes infiltration and local hemorrhage, whereas those of group C were significantly lessened.
CONCLUSION
Ulinastatin may ameliorate acute lung injury to some extent after PQ poisoning in rats by enhancing the expression of HSP70.
背景
百草枯（PQ）是一种有效的除草剂，在农业生产中广泛应用，但 PQ 中毒在人类中经常发生，靶器官为肺。临床上常根据肺部的病理变化来预测患者的严重程度和预后。本研究观察了 PQ 中毒后大鼠肺组织中热休克蛋白 70（HSP70）的表达，并探讨了乌司他丁的治疗作用。
方法
72 只成年健康 SD 大鼠随机分为对照组（A 组，n=24）、中毒组（B 组，n=24）和乌司他丁组（C 组，n=24）。B、C 组大鼠经胃内灌胃 80mg/kg PQ 建立急性 PQ 中毒大鼠模型，C 组大鼠于中毒后 30 分钟时腹腔内注射 100 000IU/kg 乌司他丁。观察肺组织中 HSP70 的表达，并比较中毒后 12、24、48 和 72 小时肺组织 W/D 值及病理变化。采用 RT-PCR 法检测肺组织中 HSP70 的表达。所有定量数据采用单因素方差分析进行处理，比较多个样本均数。
结果
与 A 组比较，B、C 组大鼠肺组织中 HSP70 的表达在各时间点均明显升高（P<0.05）。PQ 中毒大鼠肺组织病理变化包括淤血、白细胞浸润和局部出血，C 组大鼠上述病变明显减轻。
结论
乌司他丁可能通过增强 HSP70 的表达，在一定程度上减轻 PQ 中毒大鼠急性肺损伤。
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