Chattopadhyay Sunil K, Kumar Satyanshu, Kaur Ranjeet, Tandon Sudeep, Rane Shailendra
Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow-226015, India.
Biomed Chromatogr. 2009 Apr;23(4):340-56. doi: 10.1002/bmc.1121.
A sensitive liquid chromatography/electrospray ionization tandem mass spectrometric (LC/ESI-MS/MS) method was developed for the identification and quantification of two antihepatotoxic coumarinolignoids cleomiscosin A and cleomiscosin B in different extracts of the seeds of Cleome viscosa. The separation of cleomiscosin A and cleomiscosin B was achieved on an RP(18) column using a solvent system consisting of a mixture of acetonitrile-methanol (1:2, v/v) and acetonitrile-water-formic acid (5:95:0.3, v/v) as a mobile phase in a gradient elution mode. A multiple-reaction monitoring (MRM) method was developed for quantification of cleomiscosin A and cleomiscosin B in the seed extracts of Cleome viscosa. On the basis of signal-to-noise ratio of 3, the limit of detection in MRM mode for cleomiscosin A and cleomiscosin B were 1.0 and 4.0 ng/mL respectively. The method was validated in terms of linearity, accuracy and precision for 6 days. The method developed was found to be useful for identification and quantification of cleomiscosin A and cleomiscosin B in the different extracts of the seeds of Cleome viscosa.
建立了一种灵敏的液相色谱/电喷雾电离串联质谱（LC/ESI-MS/MS）方法，用于鉴定和定量粘毛醉蝶花种子不同提取物中的两种抗肝毒性香豆素木脂素——醉蝶花素A和醉蝶花素B。在RP(18)柱上，以乙腈-甲醇（1:2，v/v）和乙腈-水-甲酸（5:95:0.3，v/v）的混合溶剂体系作为流动相，采用梯度洗脱模式实现了醉蝶花素A和醉蝶花素B的分离。开发了一种多反应监测（MRM）方法，用于定量粘毛醉蝶花种子提取物中的醉蝶花素A和醉蝶花素B。基于3的信噪比，MRM模式下醉蝶花素A和醉蝶花素B的检测限分别为1.0和4.0 ng/mL。该方法在6天内进行了线性、准确度和精密度方面的验证。所建立的方法被发现可用于鉴定和定量粘毛醉蝶花种子不同提取物中的醉蝶花素A和醉蝶花素B。
