Kaur Ranjeet, Kumar Satyanshu, Chatterjee Arnab, Chattopadhyay Sunil K
Central Institute of Medicinal and Aromatic Plants, PO CIMAP, Lucknow, India.
Biomed Chromatogr. 2010 Sep;24(9):1000-5. doi: 10.1002/bmc.1399.
A simple, rapid, accurate and reproducible reverse-phase HPLC method has been developed for simultaneous identification and quantification of three coumarinolignoids, cleomiscosin A (Cliv A), cleomiscosin B (Cliv B) and cleomiscosin C (Cliv C) in different extracts of the seeds of Cleome viscosa using photodiode array detection at 326 nm. Cliv A, B and C were separated on a Waters symmetry C(18) column (250 x 4.6 mm, 5 microm) using the solvent system consisting of a mixture of acetonitrile : methanol (1:2 v/v) and water : acetic acid (99.5:0.5 v/v) as a mobile phase in a gradient elution mode. The calibration curves were linear in the concentration ranges 15-200, 10-80 and 15-180 microg/mL for Cliv A, Cliv B and Cliv C, respectively. The limits of detection and quantification for Cliv A, Cliv B and Cliv C were 15 and 20 microg/mL, 10 and 15 microg/mL and 15 and 20 microg/mL, respectively. The intra-day and inter-day precisions were 2.08 and 0.93% for Cliv A , 1.22 and 0.39% for Cliv B and 1.29 and 0.23 for Cliv C respectively. The developed HPLC method was used to identify and quantify Cliv A, Cliv B and Cliv C in different extracts of seed of Cleome viscosa.
已开发出一种简单、快速、准确且可重现的反相高效液相色谱法，用于同时鉴定和定量黄花稔种子不同提取物中的三种香豆素木脂素，即粘毛决明素A（Cliv A）、粘毛决明素B（Cliv B）和粘毛决明素C（Cliv C），采用光电二极管阵列检测，检测波长为326 nm。使用由乙腈:甲醇（1:2 v/v）和水:乙酸（99.5:0.5 v/v）的混合物组成的溶剂系统作为流动相，在梯度洗脱模式下，在Waters symmetry C(18)柱（250×4.6 mm，5 µm）上分离Cliv A、B和C。Cliv A、Cliv B和Cliv C的校准曲线在浓度范围分别为15 - 200、10 - 80和15 - 180 μg/mL时呈线性。Cliv A、Cliv B和Cliv C的检测限和定量限分别为15和20 μg/mL、10和15 μg/mL以及15和20 μg/mL。Cliv A的日内和日间精密度分别为2.08%和0.93%，Cliv B的分别为1.22%和0.39%，Cliv C的分别为1.29%和0.23%。所开发的高效液相色谱法用于鉴定和定量黄花稔种子不同提取物中的Cliv A、Cliv B和Cliv C。
