Razo-Rodríguez Ana Carolina, Chirino Yolanda I, Sánchez-González Dolores Javier, Martínez-Martínez Claudia María, Cruz Cristino, Pedraza-Chaverri José
Department of Biology, School of Chemistry, National Autonomous University of Mexico, México, DF, México.
J Med Food. 2008 Sep;11(3):582-6. doi: 10.1089/jmf.2008.0033.
cis-Diamminedichloroplatinum (II) (cisplatin) is an effective chemotherapeutic agent successfully used in the treatment of a wide range of tumors. Nevertheless, nephrotoxicity has restricted its clinical use. The use of more than a few antioxidants has shown that reactive oxygen species are involved in cisplatin-induced nephrotoxicity. In the present work the effect of garlic powder, a recognized antioxidant, on cisplatin-induced nephrotoxicity and oxidative and nitrosative stress was studied. Rats were fed with a 2% garlic powder diet for 4 weeks. A single injection of cisplatin (7.5 mg/kg) induced tubular damage and an increase in the following markers of renal injury 3 days later: blood urea nitrogen, serum creatinine, and urinary excretion of N-acetyl-beta-D-glucosaminidase. The cisplatin injection also increased 3-nitrotyrosine and 4-hydroxy-2-nonenal immunostaining in renal cortex and medulla. It was found that the garlic powder feeding was able to prevent by 40-59% the alterations in the markers of renal injury studied, by 33% the histological damage, and by 38-75% the increase in markers of oxidative and nitrosative stress. It is concluded that the ability of garlic powder to ameliorate cisplatin-induced renal injury is associated with its antioxidant properties. Our data support the use of garlic powder as a renoprotective agent.
顺二氯二氨合铂（II）（顺铂）是一种有效的化疗药物，已成功用于治疗多种肿瘤。然而，肾毒性限制了其临床应用。使用多种抗氧化剂的研究表明，活性氧参与了顺铂诱导的肾毒性。在本研究中，我们研究了公认的抗氧化剂大蒜粉对顺铂诱导的肾毒性以及氧化应激和亚硝化应激的影响。给大鼠喂食含2%大蒜粉的饲料，持续4周。单次注射顺铂（7.5mg/kg）3天后可导致肾小管损伤，并使以下肾损伤标志物增加：血尿素氮、血清肌酐和N-乙酰-β-D-氨基葡萄糖苷酶的尿排泄量。顺铂注射还增加了肾皮质和髓质中3-硝基酪氨酸和4-羟基-2-壬烯醛的免疫染色。结果发现，喂食大蒜粉能够使所研究的肾损伤标志物的改变减少40%-59%，使组织学损伤减少33%，并使氧化应激和亚硝化应激标志物的增加减少38%-75%。得出的结论是，大蒜粉改善顺铂诱导的肾损伤的能力与其抗氧化特性有关。我们的数据支持将大蒜粉用作肾脏保护剂。
