Fujii Etsuko, Suzuki Masami, Matsubara Koichi, Watanabe Miho, Chen Yu Jau, Adachi Kenji, Ohnishi Yasuyuki, Tanigawa Manabu, Tsuchiya Masayuki, Tamaoki Norikazu
PharmaLogicals Research Pte. Ltd., Singapore.
Pathol Int. 2008 Sep;58(9):559-67. doi: 10.1111/j.1440-1827.2008.02271.x.
Immunodeficient mice are widely used for xenografts of human cells and tissue. The purpose of the present study was to investigate the characteristics of xenograft human tumor models using engraftment of various non-hematopoietic tumors in the NOD/SCID/gamma(c) (null) mouse. For tumor models, human solid tumor tissues were serially passaged three or more times to establish tissue lines. A total of 326 fresh tumor specimens, mainly gastrointestinal and female genital tissue, were engrafted with 54 established tissue lines. The types of tissue lines varied and included tumor tissue of both epithelial and mesenchymal origin. In some cases the original surgical specimen was replaced with large mononuclear cells. In the established tumor tissue lines, differentiation and tumor structure were similar to that of the original surgical specimen. The interstitium of the xenograft tissue in the tissue lines was relatively well preserved although slightly decreased and replaced by host tissue. These results indicate that human solid tumors can be successfully engrafted into the NOD/SCID/gamma(c) (null) mouse and that tissue lines with the characteristics of the original tumors can be established. Investigators in the field of tumor research will benefit from the availability of tissue lines that allow the establishment of more relevant in vivo human tissue models.
免疫缺陷小鼠被广泛用于人类细胞和组织的异种移植。本研究的目的是利用各种非造血肿瘤在NOD/SCID/γ(c)（缺失）小鼠体内的植入，研究异种移植人类肿瘤模型的特征。对于肿瘤模型，人类实体瘤组织连续传代三次或更多次以建立组织系。总共326个新鲜肿瘤标本，主要是胃肠道和女性生殖组织，用54个已建立的组织系进行植入。组织系的类型各不相同，包括上皮和间充质来源的肿瘤组织。在某些情况下，原始手术标本被大单核细胞取代。在已建立的肿瘤组织系中，分化和肿瘤结构与原始手术标本相似。组织系中异种移植组织的间质相对保存完好，尽管略有减少并被宿主组织取代。这些结果表明，人类实体瘤可以成功植入NOD/SCID/γ(c)（缺失）小鼠体内，并且可以建立具有原始肿瘤特征的组织系。肿瘤研究领域的研究人员将受益于这些组织系的可用性，这些组织系有助于建立更相关的体内人类组织模型。
