与藤黄微球菌的多核苷酸磷酸化酶相关的脱氧腺苷酸激酶活性。

A deoxyadenylate kinase activity associated with polynucleotide phosphorylase from Micrococcus luteus.

作者信息

Craine J E, Klee C B

出版信息

Nucleic Acids Res. 1976 Nov;3(11):2923-8. doi: 10.1093/nar/3.11.2923.

DOI:10.1093/nar/3.11.2923

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC343142/

Abstract

We report here the presence of two enzymatic activities associated with highly purified preparations of polynucleotide phosphorylase from Micrococcus luteus. The first, a nuclease activity, which is not separated from the phosphorylase on hydroxylapatite, may be due to substitution of H2O for phosphate in the phosphorolysis reaction. The second activity, a deoxyadenylate kinase, the bulk of which is not resolved from the phosphorylase using gel filtration, sucrose density gradient centrifugation, DEAE-Sephadex, or hydroxylapatite chromatography, may represent a new activity of polynucleotide phosphorylase or be due to an enzyme which is tightly bound to the phosphorylase. Several properties of the kinase are described and its possible significance with respect to the overall enzyme mechanism is discussed.

摘要

我们在此报告，从藤黄微球菌中高度纯化得到的多核苷酸磷酸化酶制剂存在两种酶活性。第一种是核酸酶活性，在羟基磷灰石上它与磷酸化酶未分离，这可能是由于在磷酸解反应中水分子替代了磷酸基团所致。第二种活性是脱氧腺苷酸激酶，使用凝胶过滤、蔗糖密度梯度离心、DEAE - 葡聚糖凝胶或羟基磷灰石色谱法，其大部分与磷酸化酶无法分离，这可能代表多核苷酸磷酸化酶的一种新活性，或者是由于一种与磷酸化酶紧密结合的酶所致。文中描述了该激酶的几个特性，并讨论了其在整个酶作用机制方面可能具有的意义。

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引用本文的文献

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Simplified methods for large scale enzymatic synthesis of oligoribonucleotides.大规模酶促合成寡核糖核苷酸的简化方法。

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本文引用的文献

1

Deoxyadenosine diphosphate as a substrate and inhibitor of polynucleotide phosphorylase of Micrococcus luteus. I. Deoxyadenosine diphosphate as a substrate for polymerization and the exchange reaction with inorganic 32 P.二磷酸脱氧腺苷作为藤黄微球菌多核苷酸磷酸化酶的底物和抑制剂。I. 二磷酸脱氧腺苷作为聚合反应的底物以及与无机32P的交换反应

J Biol Chem. 1971 Dec 25;246(24):7486-96.

2

The processive degradation of individual polyribonucleotide chains. I. Escherichia coli ribonuclease II.单个多聚核糖核苷酸链的连续降解。I. 大肠杆菌核糖核酸酶II

J Biol Chem. 1968 Mar 10;243(5):913-22.

3

Nucleoside diphosphokinase activity associated with DNA polymerases.与DNA聚合酶相关的核苷二磷酸激酶活性。

Proc Natl Acad Sci U S A. 1971 Sep;68(9):2298-302. doi: 10.1073/pnas.68.9.2298.

4

Identification of the nucleoside monophosphate end-group on the product of the polynucleotide phosphorylase reaction.多核苷酸磷酸化酶反应产物中核苷单磷酸末端基团的鉴定。

Biochem Biophys Res Commun. 1966 May 25;23(4):448-52. doi: 10.1016/0006-291x(66)90748-0.

5

Initiation of transcription is temperature-dependent in an E. coli mutant with ts adenylate kinase.在一个含有温度敏感型腺苷酸激酶的大肠杆菌突变体中，转录起始是温度依赖性的。

Biochem Biophys Res Commun. 1975 Nov 3;67(1):353-8. doi: 10.1016/0006-291x(75)90323-x.

6

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Eur J Biochem. 1975 Oct 1;58(1):87-94. doi: 10.1111/j.1432-1033.1975.tb02352.x.

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