• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

与DNA聚合酶相关的核苷二磷酸激酶活性。

Nucleoside diphosphokinase activity associated with DNA polymerases.

作者信息

Miller L K, Wells R D

出版信息

Proc Natl Acad Sci U S A. 1971 Sep;68(9):2298-302. doi: 10.1073/pnas.68.9.2298.

DOI:10.1073/pnas.68.9.2298
PMID:4332252
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC389404/
Abstract

Nucleoside diphosphokinase activity is present in highly purified preparations of DNA polymerase from Micrococcus luteus and Escherichia coli, and in a partially purified DNA polymerase from avian myeloblastosis virus. The activity is also observed in the protein fragment of molecular weight 76,000 that is produced by subtilisin cleavage of DNA polymerase I from E. coli. The NDP kinase activity in DNA polymerase preparations from M. luteus uses various ribo- and deoxyribonucleoside di- and triphosphates as substrates. The presence of this activity in preparations of DNA polymerase results in the apparent use of deoxyribonucleoside diphosphates as substrates for DNA synthesis, provided that some triphosphate is present to serve as a phosphate donor.

摘要

核苷二磷酸激酶活性存在于从藤黄微球菌和大肠杆菌高度纯化的DNA聚合酶制剂中，以及从禽成髓细胞瘤病毒部分纯化的DNA聚合酶中。在由枯草杆菌蛋白酶切割大肠杆菌DNA聚合酶I产生的分子量为76,000的蛋白质片段中也观察到该活性。藤黄微球菌DNA聚合酶制剂中的NDP激酶活性使用各种核糖和脱氧核糖核苷二磷酸及三磷酸作为底物。DNA聚合酶制剂中这种活性的存在导致在有一些三磷酸作为磷酸供体的情况下，脱氧核糖核苷二磷酸明显被用作DNA合成的底物。

相似文献

1
Nucleoside diphosphokinase activity associated with DNA polymerases.与DNA聚合酶相关的核苷二磷酸激酶活性。
Proc Natl Acad Sci U S A. 1971 Sep;68(9):2298-302. doi: 10.1073/pnas.68.9.2298.
2
Comparison of some reactions catalyzed by deoxyribonucleic acid polymerase from avian myeloblastosis virus, Escherichia coli, and Micrococcus luteus.禽成髓细胞瘤病毒、大肠杆菌和藤黄微球菌的脱氧核糖核酸聚合酶催化的一些反应的比较
Biochemistry. 1972 Feb 15;11(4):621-9. doi: 10.1021/bi00754a025.
3
The synthesis and enzymatic polymerization of nucleotides containing mercury: potential tools for nucleic acid sequencing and structural analysis.含汞核苷酸的合成与酶促聚合：核酸测序和结构分析的潜在工具。
Proc Natl Acad Sci U S A. 1973 Aug;70(8):2238-42. doi: 10.1073/pnas.70.8.2238.
4
Ultraviolet-stimulated DNA synthesis in toluenzied Escherichia coli deficient in DNA polymerase I.在缺乏DNA聚合酶I的甲苯处理的大肠杆菌中紫外线刺激的DNA合成
Proc Natl Acad Sci U S A. 1973 Jan;70(1):129-33. doi: 10.1073/pnas.70.1.129.
5
The differential effect of magnesium and manganese ions on the synthesis of poly (dGd.C) and Micrococcus luteus DNA by Micrococcus luteus DNA polymerase.镁离子和锰离子对藤黄微球菌DNA聚合酶合成聚（dGd.C）和藤黄微球菌DNA的差异效应。
J Mol Biol. 1971 Oct 14;61(1):1-23. doi: 10.1016/0022-2836(71)90203-8.
6
Purification of the DNA polymerase of avian myeloblastosis virus.禽成髓细胞瘤病毒DNA聚合酶的纯化
Biochim Biophys Acta. 1971 Sep 24;246(3):365-83. doi: 10.1016/0005-2787(71)90773-8.
7
Polymerization of deoxyribonucleoside diphosphates with an enzyme from an Escherichia coli mutant lacking deoxyribonucleic acid polymerase activity.利用来自缺乏脱氧核糖核酸聚合酶活性的大肠杆菌突变体的一种酶使脱氧核糖核苷二磷酸发生聚合反应。
J Biol Chem. 1971 Mar 25;246(6):1780-4.
8
Different mode of action of ribonuclease H in purified alpha and alpha beta ribonucleic acid-directed deoxyribonucleic acid polymerase from avian myeloblastosis virus.来自禽成髓细胞瘤病毒的纯化α和αβ核糖核酸指导的脱氧核糖核酸聚合酶中核糖核酸酶H的不同作用模式。
J Biol Chem. 1974 Aug 25;249(16):5148-52.
9
Autoradiographic method for characterization of DNA and RNA polymerases after gel electrophoresis.凝胶电泳后用于鉴定DNA和RNA聚合酶的放射自显影方法。
Anal Biochem. 1971 Aug;42(2):509-15. doi: 10.1016/0003-2697(71)90066-2.
10
Inhibition of RNA-dependent DNA polymerase of avian myeloblastosis virus by pyran copolymer.吡喃共聚物对禽成髓细胞瘤病毒的RNA依赖性DNA聚合酶的抑制作用
Proc Natl Acad Sci U S A. 1974 Feb;71(2):367-70. doi: 10.1073/pnas.71.2.367.

引用本文的文献

1
Expression of functional proteins of cDNA encoding rice nucleoside diphosphate kinase (NDK) in Escherichia coli and organ-related alteration of NDK activities during rice seed germination (Oryza sativa L.).水稻核苷二磷酸激酶（NDK）编码cDNA功能蛋白在大肠杆菌中的表达及水稻种子萌发（Oryza sativa L.）过程中NDK活性的器官相关变化
Plant Mol Biol. 1995 Mar;27(5):1053-8. doi: 10.1007/BF00037032.
2
Mechanistic independence of avian myeloblastosis virus DNA polymerase and ribonuclease H.禽成髓细胞瘤病毒DNA聚合酶与核糖核酸酶H的机制独立性
J Virol. 1974 Dec;14(6):1494-502. doi: 10.1128/JVI.14.6.1494-1502.1974.
3
Ribonuclease H: a ubiquitous activity in virions of ribonucleic acid tumor viruses.核糖核酸酶H：在核糖核酸肿瘤病毒的病毒粒子中普遍存在的一种活性。
J Virol. 1972 Dec;10(6):1136-42. doi: 10.1128/JVI.10.6.1136-1142.1972.
4
Detergent-inhibited, heat-labile nucleoside triphosphatase in cores of avian myeloblastosis virus.禽成髓细胞瘤病毒核心中受去污剂抑制的热不稳定核苷三磷酸酶
J Virol. 1978 Nov;28(2):427-33. doi: 10.1128/JVI.28.2.427-433.1978.
5
A deoxyadenylate kinase activity associated with polynucleotide phosphorylase from Micrococcus luteus.与藤黄微球菌的多核苷酸磷酸化酶相关的脱氧腺苷酸激酶活性。
Nucleic Acids Res. 1976 Nov;3(11):2923-8. doi: 10.1093/nar/3.11.2923.
6
Deoxyribonucleic acid polymerases of Euglena gracilis. Primer-template utilization of and enzyme activities associated with the two deoxyribonucleic acid polymerases of high molecular weight.纤细裸藻的脱氧核糖核酸聚合酶。两种高分子量脱氧核糖核酸聚合酶的引物-模板利用及相关酶活性
Biochem J. 1975 Nov;151(2):239-47. doi: 10.1042/bj1510239.

本文引用的文献

1
SYNTHETIC DEOXYRIBOPOLYNUCLEOTIDES AS TEMPLATES FOR RIBONUCLEIC ACID POLYMERASE: THE FORMATION AND CHARACTERIZATION OF A RIBOPOLYNUCLEOTIDE WITH A REPEATING TRINUCLEOTIDE SEQUENCE.作为核糖核酸聚合酶模板的合成脱氧核糖多核苷酸：具有重复三核苷酸序列的核糖多核苷酸的形成与特性
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1494-501. doi: 10.1073/pnas.52.6.1494.
2
NUCLEOSIDE TRIPHOSPHATE-NUCLEOSIDE DIPHOSPHATE TRANSPHOSPHORYLASE (NUCLEOSIDE DIPHOSPHOKINASE). I. ISOLATION OF THE CRYSTALLINE ENZYME FROM BREWERS' YEAST.核苷三磷酸-核苷二磷酸转磷酸化酶（核苷二磷酸激酶）。I. 从酿酒酵母中分离结晶酶。
J Biol Chem. 1964 Jan;239:301-9.
3
THE ENZYMOLOGY OF VIRUS-INFECTED BACTERIA. V. PHOSPHORYLATION OF HYDROXYMETHYLDEOXYCYTIDINE DIPHOSPHATE AND DEOXYTHYMIDINE DIPHOSPHATE IN NORMAL AND BACTERIOPHAGE-INFECTED ESCHERICHIA COLI.病毒感染细菌的酶学。V. 正常及噬菌体感染的大肠杆菌中羟甲基脱氧胞苷二磷酸和脱氧胸苷二磷酸的磷酸化作用
Biochim Biophys Acta. 1963 Aug 20;72:647-50. doi: 10.1016/0006-3002(63)90293-2.
4
Purification and properties of deoxyribonucleic acid polymerase from Micrococcus lysodeikticus.溶壁微球菌脱氧核糖核酸聚合酶的纯化及性质
J Biol Chem. 1966 May 10;241(9):2035-41.
5
Erythrocytic nucleoside diphosphokinase. 3. Studies with free and phosphorylated enzyme and evidence for an essential thiol group.红细胞核苷二磷酸激酶。3. 游离及磷酸化酶的研究以及必需巯基的证据。
J Biol Chem. 1966 Aug 25;241(16):3838-44.
6
Erythrocytic nucleoside diphosphokinase. II. Isolation and kinetics.红细胞核苷二磷酸激酶。II. 分离与动力学
J Biol Chem. 1966 Jan 25;241(2):271-8.
7
Kinetic studies of yeast nucleoside diphosphate kinase.酵母核苷二磷酸激酶的动力学研究
Biochemistry. 1969 Feb;8(2):633-40. doi: 10.1021/bi00830a026.
8
Erythrocytic nucleoside diphosphokinase. V. Some properties and behavior of the pI 7.3 isozyme.红细胞核苷二磷酸激酶。V. 等电点7.3同工酶的一些性质和行为。
J Biol Chem. 1971 Apr 10;246(7):2258-64.
9
Physicochemical studies on polydeoxyribonucleotides containing defined repeating nucleotide sequences.对含有特定重复核苷酸序列的聚脱氧核糖核苷酸的物理化学研究。
J Mol Biol. 1970 Dec 28;54(3):465-97. doi: 10.1016/0022-2836(70)90121-x.
10
Dinucleotides as products of an exonucleolytic activity association with the Micrococcus luteus DNA polymerase.作为与藤黄微球菌DNA聚合酶相关的核酸外切酶活性产物的二核苷酸。
Proc Natl Acad Sci U S A. 1970 Jul;66(3):595-602. doi: 10.1073/pnas.66.3.595.